Inertial focusing of microparticles, bacteria, and blood in serpentine glass channels
Rodriguez-Mateos, Pablo; Ngamsom, Bongkot; Dyer, Charlotte E.; Iles, Alexander; Pamme, Nicole
Dr Charlotte Dyer C.E.Dyer@hull.ac.uk
Lecturer in Biomedical Sciences
Dr Alex Iles a.Iles@hull.ac.uk
Experimental Officer - Lab on a Chip
Early detection of pathogenic microorganisms is pivotal to diagnosis and prevention of health and safety crises. Standard methods for pathogen detection often rely on lengthy culturing procedures, confirmed by biochemical assays, leading to >24 h for a diagnosis. The main challenge for pathogen detection is their low concentration within complex matrices. Detection of blood-borne pathogens via techniques such as PCR requires an initial positive blood culture and removal of inhibitory blood components, reducing its potential as a diagnostic tool. Among different label-free microfluidic techniques, inertial focusing on microscale channels holds great promise for automation, parallelization, and passive continuous separation of particles and cells. This work presents inertial microfluidic manipulation of small particles and cells (1–10 μm) in curved serpentine glass channels etched at different depths (deep and shallow designs) that can be exploited for (1) bacteria preconcentration from biological samples and (2) bacteria-blood cell separation. In our shallow device, the ability to focus Escherichia coli into the channel side streams with high recovery (89% at 2.2× preconcentration factor) could be applied for bacteria preconcentration in urine for diagnosis of urinary tract infections. Relying on differential equilibrium positions of red blood cells and E. coli inside the deep device, 97% red blood cells were depleted from 1:50 diluted blood with 54% E. coli recovered at a throughput of 0.7 mL/min. Parallelization of such devices could process relevant volumes of 7 mL whole blood in 10 min, allowing faster sample preparation for downstream molecular diagnostics of bacteria present in bloodstream.
Rodriguez-Mateos, P., Ngamsom, B., Dyer, C. E., Iles, A., & Pamme, N. (in press). Inertial focusing of microparticles, bacteria, and blood in serpentine glass channels. ELECTROPHORESIS, https://doi.org/10.1002/elps.202100083
|Journal Article Type||Article|
|Acceptance Date||May 18, 2021|
|Online Publication Date||May 25, 2021|
|Deposit Date||May 27, 2021|
|Publicly Available Date||May 26, 2022|
|Peer Reviewed||Peer Reviewed|
|Keywords||Bacteria; Inertial; Microfluidics; Pre-concentration; Separation|
|Additional Information||Received: 2021-03-29; Accepted: 2021-05-18; Published: 2021-05-25|
This file is under embargo until May 26, 2022 due to copyright reasons.
Contact N.Pamme@hull.ac.uk to request a copy for personal use.
You might also like
FISH and chips: a review of microfluidic platforms for FISH analysis
Host-pathogen adhesion as the basis of innovative diagnostics for emerging pathogens