Jwan Oday
Development and validation of spectrophotometric method and paper-based microfluidic devices for the quantitative determination of Amoxicillin in pure form and pharmaceutical formulations
Oday, Jwan; Hadi, Hind; Hashim, Parween; Richardson, Samantha; Iles, Alexander; Pamme, Nicole
Authors
Hind Hadi
Parween Hashim
Dr Samantha Richardson Samantha.Richardson@hull.ac.uk
Lecturer in Biochemsitry
Alexander Iles
Nicole Pamme
Abstract
There is a growing need for easy-to-use, low cost and portable quantitative assays to determine active pharmaceutical ingredients in the pharmaceutical industry. Here, we developed a batch spectrophotometric method and a method employing a paper-based microfluidic device for the estimation of Amoxicillin (AMX) in pure solution and pharmaceutical preparations. The detection depends on the coupling reaction of Amoxicillin with diazotized sulfadimidine (DSDM) in an alkaline medium. The yellow azo dye reaction product was measured at λmax 425 nm and linearity was observed from 2 to 30 mg L−1 with a detection limit of 0.32 mg L−1 and a quantification limit of 1.2 mg L−1 was found. The reaction was then transferred onto the paper-based microfluidic device and a plateau change in color intensity was found above 10 mg L−1. Thus, the paper-based microfluidic device can be applied for the semi-quantitative determination of Amoxicillin in pure solution and commercial pharmaceutical products for rapid screening.
Citation
Oday, J., Hadi, H., Hashim, P., Richardson, S., Iles, A., & Pamme, N. (2024). Development and validation of spectrophotometric method and paper-based microfluidic devices for the quantitative determination of Amoxicillin in pure form and pharmaceutical formulations. Heliyon, 10(3), Article e24968. https://doi.org/10.1016/j.heliyon.2024.e24968
Journal Article Type | Article |
---|---|
Acceptance Date | Jan 17, 2024 |
Online Publication Date | Jan 22, 2024 |
Publication Date | Feb 15, 2024 |
Deposit Date | Feb 22, 2024 |
Publicly Available Date | Feb 23, 2024 |
Journal | Heliyon |
Print ISSN | 2405-8440 |
Publisher | Elsevier |
Peer Reviewed | Peer Reviewed |
Volume | 10 |
Issue | 3 |
Article Number | e24968 |
DOI | https://doi.org/10.1016/j.heliyon.2024.e24968 |
Keywords | Amoxicillin (AMX); Spectrophotometry; Diazotized sulfadimidine (DSDM); Paper-based microfluidic device; Pharmaceutical dosage forms |
Public URL | https://hull-repository.worktribe.com/output/4529784 |
Additional Information | This article is maintained by: Elsevier; Article Title: Development and validation of spectrophotometric method and paper-based microfluidic devices for the quantitative determination of Amoxicillin in pure form and pharmaceutical formulations; Journal Title: Heliyon; CrossRef DOI link to publisher maintained version: https://doi.org/10.1016/j.heliyon.2024.e24968; Content Type: article; Copyright: © 2024 The Authors. Published by Elsevier Ltd. |
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Copyright Statement
© 2024 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
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