Mengke Han
Cytoplasmic delivery of quantum dots via microelectrophoresis technique
Han, Mengke; Zhao, Jiangbo; Fabian, Joseph Mahandas; Evans, Samuel; Mustafa, Sanam; Ruan, Yinlan; Wiederman, Steven; Ebendorff-Heidepriem, Heike
Authors
Dr Jiangbo Zhao Jiangbo.Zhao@hull.ac.uk
Lecturer
Joseph Mahandas Fabian
Samuel Evans
Sanam Mustafa
Yinlan Ruan
Steven Wiederman
Heike Ebendorff-Heidepriem
Abstract
Nanoparticles with specific properties and functions have been developed for various biomedical research applications, such as in vivo and in vitro sensors, imaging agents and delivery vehicles of therapeutics. The development of an effective delivery method of nanoparticles into the intracellular environment is challenging and success in this endeavor would be beneficial to many biological studies. Here, the well-established microelectrophoresis technique was applied for the first time to deliver nanoparticles into living cells. An optimal protocol was explored to prepare semiconductive quantum dots suspensions having high monodispersity with average hydrodynamic diameter of 13.2–35.0 nm. Micropipettes were fabricated to have inner tip diameters of approximately 200 nm that are larger than quantum dots for ejection but less than 500 nm to minimize damage to the cell membrane. We demonstrated the successful delivery of quantum dots via small electrical currents (–0.2 nA) through micropipettes into the cytoplasm of living human embryonic kidney cells (roughly 20–30 μm in length) using microelectrophoresis technique. This method is promising as a simple and general strategy for delivering a variety of nanoparticles into the cellular environment.
Citation
Han, M., Zhao, J., Fabian, J. M., Evans, S., Mustafa, S., Ruan, Y., Wiederman, S., & Ebendorff-Heidepriem, H. (2021). Cytoplasmic delivery of quantum dots via microelectrophoresis technique. ELECTROPHORESIS, 42(11), 1247-1254. https://doi.org/10.1002/elps.202000388
Journal Article Type | Article |
---|---|
Acceptance Date | Feb 17, 2021 |
Publication Date | Jun 1, 2021 |
Deposit Date | Apr 12, 2022 |
Publicly Available Date | Jul 6, 2022 |
Journal | Electrophoresis |
Print ISSN | 0173-0835 |
Electronic ISSN | 1522-2683 |
Publisher | Wiley-VCH Verlag |
Peer Reviewed | Peer Reviewed |
Volume | 42 |
Issue | 11 |
Pages | 1247-1254 |
DOI | https://doi.org/10.1002/elps.202000388 |
Keywords | Intracellular delivery, Microelectrophoresis, Nanoparticles, Quantum dots, Biosensor |
Public URL | https://hull-repository.worktribe.com/output/3969409 |
Files
Accepted manuscript
(850 Kb)
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Copyright Statement
© John Wiley & Sons. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.
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