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Cytoplasmic delivery of quantum dots via microelectrophoresis technique

Han, Mengke; Zhao, Jiangbo; Fabian, Joseph Mahandas; Evans, Samuel; Mustafa, Sanam; Ruan, Yinlan; Wiederman, Steven; Ebendorff-Heidepriem, Heike

Authors

Mengke Han

Joseph Mahandas Fabian

Samuel Evans

Sanam Mustafa

Yinlan Ruan

Steven Wiederman

Heike Ebendorff-Heidepriem



Abstract

Nanoparticles with specific properties and functions have been developed for various biomedical research applications, such as in vivo and in vitro sensors, imaging agents and delivery vehicles of therapeutics. The development of an effective delivery method of nanoparticles into the intracellular environment is challenging and success in this endeavor would be beneficial to many biological studies. Here, the well-established microelectrophoresis technique was applied for the first time to deliver nanoparticles into living cells. An optimal protocol was explored to prepare semiconductive quantum dots suspensions having high monodispersity with average hydrodynamic diameter of 13.2–35.0 nm. Micropipettes were fabricated to have inner tip diameters of approximately 200 nm that are larger than quantum dots for ejection but less than 500 nm to minimize damage to the cell membrane. We demonstrated the successful delivery of quantum dots via small electrical currents (–0.2 nA) through micropipettes into the cytoplasm of living human embryonic kidney cells (roughly 20–30 μm in length) using microelectrophoresis technique. This method is promising as a simple and general strategy for delivering a variety of nanoparticles into the cellular environment.

Citation

Han, M., Zhao, J., Fabian, J. M., Evans, S., Mustafa, S., Ruan, Y., Wiederman, S., & Ebendorff-Heidepriem, H. (2021). Cytoplasmic delivery of quantum dots via microelectrophoresis technique. ELECTROPHORESIS, 42(11), 1247-1254. https://doi.org/10.1002/elps.202000388

Journal Article Type Article
Acceptance Date Feb 17, 2021
Publication Date Jun 1, 2021
Deposit Date Apr 12, 2022
Publicly Available Date Jul 6, 2022
Journal Electrophoresis
Print ISSN 0173-0835
Electronic ISSN 1522-2683
Publisher Wiley-VCH Verlag
Peer Reviewed Peer Reviewed
Volume 42
Issue 11
Pages 1247-1254
DOI https://doi.org/10.1002/elps.202000388
Keywords Intracellular delivery, Microelectrophoresis, Nanoparticles, Quantum dots, Biosensor
Public URL https://hull-repository.worktribe.com/output/3969409

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Copyright Statement
© John Wiley & Sons. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.





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