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Phosphatidylglycerol lipids enhance folding of an α helical membrane protein

Seddon, Annela M.; Lorch, Mark; Ces, Oscar; Templer, Richard H.; Macrae, Fiona; Booth, Paula J.


Annela M. Seddon

Oscar Ces

Richard H. Templer

Fiona Macrae

Paula J. Booth


Membrane lipids are increasingly being recognised as active participants in biological events. The precise roles that individual lipids or global properties of the lipid bilayer play in the folding of membrane proteins remain to be elucidated, Here, we find a significant effect of phosphatidylglycerol (PG) on the folding of a trimeric α helical membrane protein from Escherichia coli diacylglycerol kinase. Both the rate and the yield of folding are increased by increasing the amount of PG in lipid vesicles. Moreover, there is a direct correlation between the increase in yield and the increase in rate; thus, folding becomes more efficient in terms of speed and productivity. This effect of PG seems to be a specific requirement for this lipid, rather than a charge effect. We also find an effect of single-chain lyso lipids in decreasing the rate and yield of folding. We compare this to our previous work in which lyso lipids increased the rate and yield of another membrane protein, bacteriorhodopsin. The contrasting effect of lyso lipids on the two proteins can be explained by the different folding reaction mechanisms and key folding steps involved. Our findings provide information on the lipid determinants of membrane protein folding.


Seddon, A. M., Lorch, M., Ces, O., Templer, R. H., Macrae, F., & Booth, P. J. (2008). Phosphatidylglycerol lipids enhance folding of an α helical membrane protein. Journal of Molecular Biology, 380(3), 548-556.

Journal Article Type Article
Acceptance Date Jul 11, 2008
Publication Date Jul 11, 2008
Print ISSN 0022-2836
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 380
Issue 3
Pages 548-556
Keywords membrane protein folding headgroup interactions phosphatidylglycerol lipids kinetics phospholipid-bilayer vesicles coli inner membranes escherichia-coli diacylglycerol kinase sn-1,2-diacylglycerol kinase acidic phospholipids nonbilayer lipids procoat prot
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