Dr Pedro Beltran-Alvarez P.Beltran-Alvarez@hull.ac.uk
Senior Lecturer in Health and Climate Change and Programme co-Director of the MSc Health and Climate Change
Identification of N-terminal protein acetylation and arginine methylation of the voltage-gated sodium channel in end-stage heart failure human heart
Beltran-Alvarez, Pedro; Tarradas, Anna; Chiva, Cristina; Pérez-Serra, Alexandra; Batlle, Montserrat; Pérez-Villa, Félix; Schulte, Uwe; Sabidó, Eduard; Brugada, Ramon; Pagans, Sara
Authors
Anna Tarradas
Cristina Chiva
Alexandra Pérez-Serra
Montserrat Batlle
Félix Pérez-Villa
Uwe Schulte
Eduard Sabidó
Ramon Brugada
Sara Pagans
Abstract
The α subunit of the cardiac voltage-gated sodium channel, Naᵥ1.5, provides the rapid sodium inward current that initiates cardiomyocyte action potentials. Here, we analyzed for the first time the post-translational modifications of Naᵥ1.5 purified from end-stage heart failure human cardiac tissue. We identified R526 methylation as the major post-translational modification of any Naᵥ1.5 arginine or lysine residue. Unexpectedly, we found that the N terminus of Naᵥ1.5 was: 1) devoid of the initiation methionine, and 2) acetylated at the resulting initial alanine residue. This is the first evidence for N-terminal acetylation in any member of the voltage-gated ion channel superfamily. Our results open the door to explore Naᵥ1.5 N-terminal acetylation and arginine methylation levels as drivers or markers of end-stage heart failure.
Citation
Beltran-Alvarez, P., Tarradas, A., Chiva, C., Pérez-Serra, A., Batlle, M., Pérez-Villa, F., Schulte, U., Sabidó, E., Brugada, R., & Pagans, S. (2014). Identification of N-terminal protein acetylation and arginine methylation of the voltage-gated sodium channel in end-stage heart failure human heart. Journal of Molecular and Cellular Cardiology, 76, 126-129. https://doi.org/10.1016/j.yjmcc.2014.08.014
Acceptance Date | Aug 15, 2014 |
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Online Publication Date | Aug 27, 2014 |
Publication Date | Nov 1, 2014 |
Deposit Date | Mar 10, 2016 |
Publicly Available Date | Mar 10, 2016 |
Journal | Journal of molecular and cellular cardiology |
Print ISSN | 0022-2828 |
Publisher | Elsevier |
Peer Reviewed | Peer Reviewed |
Volume | 76 |
Pages | 126-129 |
DOI | https://doi.org/10.1016/j.yjmcc.2014.08.014 |
Keywords | Voltage-gated sodium channel, Proteomics, Post-translational modification, N-terminal acetylation, Arginine methylation, Heart failure |
Public URL | https://hull-repository.worktribe.com/output/412671 |
Publisher URL | http://www.sciencedirect.com/science/article/pii/S0022282814002612 |
Additional Information | Author's accepted manuscript of article published in: Journal of molecular and cellular cardiology, 2014, v.76 |
Contract Date | Mar 10, 2016 |
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Copyright Statement
© 2014, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/
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