Lewis J. White
Two closely related ureotelic fish species of the genus Alcolapia express different levels of ammonium transporters in gills
White, Lewis J.; Rose, Matthew; Lawson, Michael; Joyce, Domino; Smith, Alan M.; Thomas, Gavin H.; Dasmahapatra, Kanchon K.; Pownall, Mary E.
Authors
Matthew Rose
Michael Lawson
Dr Domino Joyce D.Joyce@hull.ac.uk
Senior Lecturer
Alan M. Smith
Gavin H. Thomas
Kanchon K. Dasmahapatra
Mary E. Pownall
Abstract
Most fish excrete their nitrogenous waste across the gills as ammonia through the activity of the Rhesus glycoprotein ammonium transporters. In contrast, fish of the subgenus Alcolapia (Oreochromis) are the only vertebrates that survive the extreme conditions of the soda lakes of Natron and Magadi in East Africa and have evolved adaptations to the highly alkaline waters including the ability to excrete their nitrogenous waste as urea. Nevertheless, Alcolapia retain the Rhesus glycoprotein genes in their genomes and using two heterologous expression systems, we demonstrate that Alcolapia Rhbg is capable of moving ammonia. Comparing ammonia and urea excretion from two closely related Alcolapia species from the same aquarium, we found that while Alcolapia grahami remains fully ureotelic after many generations in lab conditions, Alcolapia alcalica excretes some of its nitrogenous waste as ammonia. Using in situ hybridisation, we demonstrate robust, localised gene expression of Rhbg, rhcg1 and rhcg2 in the gill tissue in both A. alcalica embryos and adults, similar to that in other ammoniotelic fish. In contrast, the expression of these genes in A. grahami gills is much lower than in A. alcalica, suggesting the rapid evolution of a molecular mechanism underlying the complete ureotelism of A. grahami.
Citation
White, L. J., Rose, M., Lawson, M., Joyce, D., Smith, A. M., Thomas, G. H., Dasmahapatra, K. K., & Pownall, M. E. (2022). Two closely related ureotelic fish species of the genus Alcolapia express different levels of ammonium transporters in gills. Biology Open, 11(11), Article bio059575. https://doi.org/10.1242/bio.059575
Journal Article Type | Article |
---|---|
Acceptance Date | Oct 10, 2022 |
Online Publication Date | Nov 7, 2022 |
Publication Date | 2022-11 |
Deposit Date | Dec 21, 2022 |
Publicly Available Date | Jan 3, 2023 |
Journal | Biology open |
Electronic ISSN | 2046-6390 |
Publisher | The Company of Biologists |
Peer Reviewed | Peer Reviewed |
Volume | 11 |
Issue | 11 |
Article Number | bio059575 |
DOI | https://doi.org/10.1242/bio.059575 |
Keywords | Extremophile; Ureotelic; Rhesus proteins; Amt/Mep; Rhbg |
Public URL | https://hull-repository.worktribe.com/output/4132679 |
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Copyright Statement
© 2022. Published by The Company of Biologists Ltd
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.
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