Directed assembly of yeast cells into living yeastosomes by microbubble templating

Brandy, Marie-Laure; Cayre, Olivier J.; Fakhrullin, Rawil F.; Velev, Orlin D.; Paunov, Vesselin N.

Abstract

We report the fabrication of yeastosomes—novel multicellular assemblies, which consist of a spherical monolayer of living yeast cells held together by colloid interactions. Our method is based on templating of microbubbles with cells coated with cationic polyelectrolyte and the layer-by-layer technique. We show the mechanism of formation of yeastosomes which involves a gradual dissolution of the air-cores and their infusion with water. We demonstrate that the cells remain viable in the yeastosome structures. Yeastosomes and similar structures may find applications in the development of novel symbiotic biostructures, artificial multicellular organisms and in tissue engineering. The unusual structure of these multicellular assemblies resembles the primitive forms of multicellular species, like Volvox, and other algae and could be regarded as one possible stage of the evolutionary development of multicellularity.

Citation

Brandy, M., Cayre, O. J., Fakhrullin, R. F., Velev, O. D., & Paunov, V. N. (2010). Directed assembly of yeast cells into living yeastosomes by microbubble templating. Soft matter, 6(15), 3494-3498. https://doi.org/10.1039/c0sm00003e

• Journal Article Type: Article
• Acceptance Date: Apr 1, 2010
• Online Publication Date: May 17, 2010
• Publication Date: Aug 7, 2010
• Journal: SOFT MATTER
• Print ISSN: 1744-683X
• Electronic ISSN: 1744-6848
• Publisher: Royal Society of Chemistry
• Peer Reviewed: Peer Reviewed
• Volume: 6
• Issue: 15
• Pages: 3494-3498
• DOI: https://doi.org/10.1039/c0sm00003e
• Keywords: Yeastosomes; Microbubbles; Directed assembly; Polyelectrolytes; Multicellularity; Templating; Cellosomes
• Public URL: https://hull-repository.worktribe.com/output/424049