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Does soluble intercellular adhesion molecule-1 (ICAM-1) affect neutrophil activation and adhesion following ischaemia-reperfusion?

Spark, JI; Scott, DJA; Chetter, IC; Guillou, PJ; Kester, RC; Spark, J.I; Scott, D.J.A; Chetter, I.C; Guillou, P.J; Kester, R.C

Authors

JI Spark

DJA Scott

IC Chetter

PJ Guillou

RC Kester

J.I Spark

D.J.A Scott

P.J Guillou

R.C Kester



Abstract

Objective: to examine the effect of reperfusion plasma and sICAM-1 on neutrophil integrin expression and neutrophil adhesion to determine if sICAM-1 has a potential role in the regulation of neutrophil adhesion. Materials: twenty-seven patients, 17 men and 10 women undergoing femorodistal surgery. Blood was taken preoperatively and from the femoral vein following the release of the cross-clamp. Neutrophils were obtained from five volunteers and incubated with phosphate buffered saline (PBS), preoperative plasma or reperfusion plasma with and without sICAM-1. Neutrophil expression of CD11b and adhesion were measured. Main results: neutrophil CD11b expression did not change following incubation in the three media. Neutrophil adhesion increased significantly following exposure to reperfusion plasma compared to PBS or preoperative plasma (45.5 adhesion vs. 12.75%, p < 0.01 Mann-Whitney U-test). Soluble ICAM-1 decreased CD11b expression and adhesion in neutrophils exposed to reperfusion plasma only (CD11b expression fell from 15.9 to 3.4 mcf, p < 0.01 Mann-Whitney U-test and adhesion fell to 11.6% cells adhered, p < 0.01). Conclusion: an increase in CD11b expression is not required for an increase in neutrophil adhesion. The change in neutrophil adhesion produced by reperfusion plasma can be blocked by sICAM-1. Soluble ICAM-1 may have a physiological role in the regulation of neutrophil adhesion.

Citation

Spark, J., Scott, D., Chetter, I., Guillou, P., & Kester, R. (1999). Does soluble intercellular adhesion molecule-1 (ICAM-1) affect neutrophil activation and adhesion following ischaemia-reperfusion?. European journal of vascular and endovascular surgery : the official journal of the European Society for Vascular Surgery, 17(2), 115-120. https://doi.org/10.1053/ejvs.1998.0710

Journal Article Type Article
Acceptance Date Aug 11, 1998
Publication Date 1999-02
Print ISSN 1078-5884
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 17
Issue 2
Pages 115-120
DOI https://doi.org/10.1053/ejvs.1998.0710
Keywords Surgery; Cardiology and Cardiovascular Medicine
Public URL https://hull-repository.worktribe.com/output/432650