The regulation of neutrophil activation and adhesion during femorodistal bypass surgery

Spark, J.I.; Chetter, I.C.; Kester, R.C.; Guillou, P.J.; Scott, D.J.A.

Abstract

Objective:To determine the effect that revascularising chronic critically ischaemic legs has no neutrophil activation and adhesion.Design:Prospective clinical study.Setting:University Hospital.Materials:Twenty-five patients, 16 men and nine women undergoing femorodistal surgery.Chief outcome measures:Venous blood assays for neutrophils expression of CD11b, neutrophil adhesion, and the plasma concentration of the shed endothelial adhesion receptor, soluble intracellular adhesion molecule 1 (sICAM-1). Urinary microalbuminaemia was measured and expressed as an albumin/creatinine ratio (ACR), as a marker of vascular permeability and plasma neutrophil elastase as evidence of neutrophil activation. Venous blood was taken preoperatively, during surgery and for the first 7 days postoperatively.Main results:Neutrophil CD11b expression fell following reperfusion of the limb (21.4 mcf to 9.7 mcf, p<0.02 Mann Whitney U-test) as did neutrophil adhesion (preop. 75% adhesion, postop. 28% p<0.01). However, the plasma elastase levels rose from 95 μg/l to 345 μg/l at 4 h and the ACR increased from 5.3 mg/ml to 304.2 mg/ml. The concentration of sICAM-1 fell following reperfusion (p<0.04).Conclusion:The change in CD11b, sICAM-1 and adhesion may represent a normal immunological response to ischaemia/reperfusion.

Citation

Spark, J., Chetter, I., Kester, R., Guillou, P., & Scott, D. (1997). The regulation of neutrophil activation and adhesion during femorodistal bypass surgery. European journal of vascular and endovascular surgery : the official journal of the European Society for Vascular Surgery, 13(4), 407-412. https://doi.org/10.1016/s1078-5884%2897%2980085-8

• Journal Article Type: Article
• Acceptance Date: Apr 30, 1997
• Publication Date: Apr 30, 1997
• Journal: European Journal of Vascular and Endovascular Surgery
• Print ISSN: 1078-5884
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 13
• Issue: 4
• Pages: 407-412
• DOI: https://doi.org/10.1016/s1078-5884%2897%2980085-8
• Keywords: Surgery; Cardiology and Cardiovascular Medicine
• Public URL: https://hull-repository.worktribe.com/output/432655