Expression and function of transient receptor potential channels in the female bovine reproductive tract

Ghavideldarestani, Maryam; Atkin, Stephen L.; Leese, Henry J.; Sturmey, Roger G.

Abstract

© 2016 Elsevier Inc. The epithelium lining the oviduct is critical for early reproductive events, many of which are mediated via intracellular calcium ions. Despite this, little is known about the regulation of calcium homeostasis in the oviductal epithelium. Epithelial transient receptor potential channels (TRPCs) modulate calcium flux in other tissues, and their expression and functional regulation have therefore been examined using the bovine oviduct as a model for the human. The effects of FSH, LH, 17β-estradiol, and progesterone on TRPCs expression and intracellular calcium flux were determined. Transient receptor potential channels 1, 2, 3, 4, and 6 were expressed in the bovine reproductive tract, and their gene expression varied throughout the estrous cycle. In more detailed studies undertaken on TRPC1 and 6, we show that protein expression varied through the estrus cycle; specifically, 17β-estradiol, FSH, and LH individually and in combination upregulated TRPC1 and 6 expression in cultured bovine oviduct epithelial cells although progesterone antagonized these effects. Functional studies showed changes in calcium mobilization in bovine oviduct epithelial cells were dependent on TRPCs. In conclusion, TRPC1, 2, 3, 4, and 6 are present in the epithelium lining the bovine oviduct, and TRPC1 and 6 vary through the estrous cycle suggesting an important role in early reproductive function.

Citation

Ghavideldarestani, M., Atkin, S. L., Leese, H. J., & Sturmey, R. G. (2016). Expression and function of transient receptor potential channels in the female bovine reproductive tract. Theriogenology, 86(2), 551-561. https://doi.org/10.1016/j.theriogenology.2016.02.005

• Journal Article Type: Article
• Acceptance Date: Feb 6, 2016
• Online Publication Date: Feb 15, 2016
• Publication Date: 2016-07
• Deposit Date: Apr 5, 2016
• Publicly Available Date: Apr 5, 2016
• Journal: Theriogenology
• Print ISSN: 0093-691X
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 86
• Issue: 2
• Pages: 551-561
• DOI: https://doi.org/10.1016/j.theriogenology.2016.02.005
• Keywords: TRPC channels, Sex hormones, Calcium, Epithelium, Oviduct, Bovine
• Public URL: https://hull-repository.worktribe.com/output/435072
• Publisher URL: http://www.sciencedirect.com/science/article/pii/S0093691X16000662
• Additional Information: This article is maintained by: Elsevier; Article Title: Expression and function of transient receptor potential channels in the female bovine reproductive tract; Journal Title: Theriogenology; CrossRef DOI link to publisher maintained version: http://dx.doi.org/10.1016/j.theriogenology.2016.02.005; Content Type: article; Copyright: © 2016 Elsevier Inc. All rights reserved.
• Contract Date: Apr 5, 2016