Dr Mauro Rinaldi M.Rinaldi@hull.ac.uk
Lecturer in Biotechnology and Biochemistry
Alternative metabolic pathways and strategies to high-titre terpenoid production in: Escherichia coli
Rinaldi, Mauro A.; Ferraz, Clara A.; Scrutton, Nigel S.
Authors
Clara A. Ferraz
Nigel S. Scrutton
Abstract
Covering: up to 2021 Terpenoids are a diverse group of chemicals used in a wide range of industries. Microbial terpenoid production has the potential to displace traditional manufacturing of these compounds with renewable processes, but further titre improvements are needed to reach cost competitiveness. This review discusses strategies to increase terpenoid titres in Escherichia coli with a focus on alternative metabolic pathways. Alternative pathways can lead to improved titres by providing higher orthogonality to native metabolism that redirects carbon flux, by avoiding toxic intermediates, by bypassing highly-regulated or bottleneck steps, or by being shorter and thus more efficient and easier to manipulate. The canonical 2-C-methyl-d-erythritol 4-phosphate (MEP) and mevalonate (MVA) pathways are engineered to increase titres, sometimes using homologs from different species to address bottlenecks. Further, alternative terpenoid pathways, including additional entry points into the MEP and MVA pathways, archaeal MVA pathways, and new artificial pathways provide new tools to increase titres. Prenyl diphosphate synthases elongate terpenoid chains, and alternative homologs create orthogonal pathways and increase product diversity. Alternative sources of terpenoid synthases and modifying enzymes can also be better suited for E. coli expression. Mining the growing number of bacterial genomes for new bacterial terpenoid synthases and modifying enzymes identifies enzymes that outperform eukaryotic ones and expand microbial terpenoid production diversity. Terpenoid removal from cells is also crucial in production, and so terpenoid recovery and approaches to handle end-product toxicity increase titres. Combined, these strategies are contributing to current efforts to increase microbial terpenoid production towards commercial feasibility.
Citation
Rinaldi, M. A., Ferraz, C. A., & Scrutton, N. S. (2022). Alternative metabolic pathways and strategies to high-titre terpenoid production in: Escherichia coli. Natural Product Reports, 39(1), 90-118. https://doi.org/10.1039/d1np00025j
Journal Article Type | Review |
---|---|
Acceptance Date | Jun 22, 2021 |
Publication Date | Jan 1, 2022 |
Deposit Date | Aug 12, 2023 |
Publicly Available Date | Aug 14, 2023 |
Journal | Natural Product Reports |
Print ISSN | 0265-0568 |
Electronic ISSN | 1460-4752 |
Publisher | Royal Society of Chemistry |
Peer Reviewed | Peer Reviewed |
Volume | 39 |
Issue | 1 |
Pages | 90-118 |
DOI | https://doi.org/10.1039/d1np00025j |
Public URL | https://hull-repository.worktribe.com/output/4355455 |
Files
Published article
(1.8 Mb)
PDF
Publisher Licence URL
http://creativecommons.org/licenses/by/3.0
Copyright Statement
Creative Commons Licence: Attribution License. See: https://creativecommons.org/licenses/by/3.0/
You might also like
Bioproduction of Linalool From Paper Mill Waste
(2022)
Journal Article
Flavonoid Versus Artemisinin Anti-malarial Activity in Artemisia annua Whole-Leaf Extracts
(2019)
Journal Article
The PEX1 ATPase stabilizes PEX6 and plays essential roles in peroxisome biology
(2017)
Journal Article
Mutation of the Arabidopsis LON2 peroxisomal protease enhances pexophagy
(2014)
Journal Article
Downloadable Citations
About Repository@Hull
Administrator e-mail: repository@hull.ac.uk
This application uses the following open-source libraries:
SheetJS Community Edition
Apache License Version 2.0 (http://www.apache.org/licenses/)
PDF.js
Apache License Version 2.0 (http://www.apache.org/licenses/)
Font Awesome
SIL OFL 1.1 (http://scripts.sil.org/OFL)
MIT License (http://opensource.org/licenses/mit-license.html)
CC BY 3.0 ( http://creativecommons.org/licenses/by/3.0/)
Powered by Worktribe © 2024
Advanced Search