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A gain-of-function mutation in IAA16 confers reduced responses to auxin and abscisic acid and impedes plant growth and fertility

Rinaldi, Mauro A.; Liu, James; Enders, Tara A.; Bartel, Bonnie; Strader, Lucia C.

Authors

James Liu

Tara A. Enders

Bonnie Bartel

Lucia C. Strader



Abstract

Auxin regulates many aspects of plant development, in part, through degradation of the Aux/IAA family of transcriptional repressors. Consequently, stabilizing mutations in several Aux/IAA proteins confer reduced auxin responsiveness. However, of the 29 apparent Aux/IAA proteins in Arabidopsis thaliana, fewer than half have roles established through mutant analysis. We identified iaa16-1, a dominant gain-of-function mutation in IAA16 (At3g04730), in a novel screen for reduced root responsiveness to abscisic acid. The iaa16-1 mutation also confers dramatically reduced auxin responses in a variety of assays, markedly restricts growth of adult plants, and abolishes fertility when homozygous. We compared iaa16-1 phenotypes with those of dominant mutants defective in the closely related IAA7/AXR2, IAA14/SLR, and IAA17/AXR3, along with the more distantly related IAA28, and found overlapping but distinct patterns of developmental defects. The identification and characterization of iaa16-1 provides a fuller understanding of the IAA7/IAA14/IAA16/IAA17 clade of Aux/IAA proteins and the diverse roles of these repressors in hormone response and plant development. © 2012 Springer Science+Business Media B.V.

Citation

Rinaldi, M. A., Liu, J., Enders, T. A., Bartel, B., & Strader, L. C. (2012). A gain-of-function mutation in IAA16 confers reduced responses to auxin and abscisic acid and impedes plant growth and fertility. Plant Molecular Biology, 79(4-5), 359-373. https://doi.org/10.1007/s11103-012-9917-y

Journal Article Type Article
Acceptance Date Apr 22, 2012
Online Publication Date May 12, 2012
Publication Date Jul 1, 2012
Deposit Date Aug 12, 2023
Journal Plant Molecular Biology
Print ISSN 0167-4412
Publisher Springer
Peer Reviewed Peer Reviewed
Volume 79
Issue 4-5
Pages 359-373
DOI https://doi.org/10.1007/s11103-012-9917-y
Keywords Auxin signaling; Abscisic acid; Aux/IAA gain of function; IAA16
Public URL https://hull-repository.worktribe.com/output/4355500