Vicki E. Maltby
Genome-wide DNA methylation changes in CD19+ B cells from relapsing-remitting multiple sclerosis patients
Maltby, Vicki E.; Lea, Rodney A.; Graves, Moira C.; Sanders, Katherine A.; Benton, Miles C.; Tajouri, Lotti; Scott, Rodney J.; Lechner-Scott, Jeannette
Authors
Rodney A. Lea
Moira C. Graves
Dr Kat Sanders Katherine.Sanders@hull.ac.uk
Senior Lecturer in Clinical Anatomy
Miles C. Benton
Lotti Tajouri
Rodney J. Scott
Jeannette Lechner-Scott
Abstract
Multiple Sclerosis (MS) is an inflammatory and neurodegenerative disease of the central nervous system. The inflammatory process in MS is driven by both T and B cells and current therapies are targeted to each of these cell types. Epigenetic mechanisms may provide a valuable link between genes and environment. DNA methylation is the best studied epigenetic mechanism and is recognized as a potential contributor to MS risk. The objective of this study was to identify DNA methylation changes associated with MS in CD19+ B-cells. We performed an epigenome-wide association analysis of DNA methylation in the CD19+ B-cells from 24 patients with relapsing-remitting MS on various treatments and 24 healthy controls using Illumina 450 K arrays. A large differentially methylated region (DMR) was observed at the lymphotoxin alpha (LTA) locus. This region was hypermethylated and contains 19 differentially methylated positions (DMPs) spanning 860 bp, all of which are located within the transcriptional start site. We also observed smaller DMRs at 4 MS-associated genes: SLC44A2, LTBR, CARD11 and CXCR5. These preliminary findings suggest that B-cell specific DNA-methylation may be associated with MS risk or response to therapy, specifically at the LTA locus. Development of B-cell specific epigenetic therapies is an attractive new avenue of research in MS treatment. Further studies are now required to validate these findings and understand their functional significance.
Citation
Maltby, V. E., Lea, R. A., Graves, M. C., Sanders, K. A., Benton, M. C., Tajouri, L., …Lechner-Scott, J. (2018). Genome-wide DNA methylation changes in CD19+ B cells from relapsing-remitting multiple sclerosis patients. Scientific reports, 8, Article 17418. https://doi.org/10.1038/s41598-018-35603-0
Journal Article Type | Article |
---|---|
Acceptance Date | Oct 17, 2018 |
Online Publication Date | Nov 27, 2018 |
Publication Date | 2018 |
Deposit Date | Aug 23, 2023 |
Publicly Available Date | Sep 1, 2023 |
Journal | Scientific Reports |
Print ISSN | 2045-2322 |
Electronic ISSN | 2045-2322 |
Publisher | Nature Publishing Group |
Peer Reviewed | Peer Reviewed |
Volume | 8 |
Article Number | 17418 |
DOI | https://doi.org/10.1038/s41598-018-35603-0 |
Public URL | https://hull-repository.worktribe.com/output/4366544 |
Files
Published article
(1.4 Mb)
PDF
Publisher Licence URL
http://creativecommons.org/licenses/by/4.0
Copyright Statement
© The Author(s) 2018
You might also like
Personal autonomy and self-determination are crucial for professionalism in healthcare
(2023)
Journal Article
Downloadable Citations
About Repository@Hull
Administrator e-mail: repository@hull.ac.uk
This application uses the following open-source libraries:
SheetJS Community Edition
Apache License Version 2.0 (http://www.apache.org/licenses/)
PDF.js
Apache License Version 2.0 (http://www.apache.org/licenses/)
Font Awesome
SIL OFL 1.1 (http://scripts.sil.org/OFL)
MIT License (http://opensource.org/licenses/mit-license.html)
CC BY 3.0 ( http://creativecommons.org/licenses/by/3.0/)
Powered by Worktribe © 2024
Advanced Search