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Nuclear Drosophila CerS Schlank regulates lipid homeostasis via the homeodomain, independent of the lag1p motif

Voelzmann, André; Wulf, Anna Lena; Eckardt, Franka; Thielisch, Melanie; Brondolin, Mirco; Pesch, Yanina Yasmin; Sociale, Mariangela; Bauer, Reinhard; Hoch, Michael

Authors

Anna Lena Wulf

Franka Eckardt

Melanie Thielisch

Mirco Brondolin

Yanina Yasmin Pesch

Mariangela Sociale

Reinhard Bauer

Michael Hoch



Abstract

Drosophila Ceramide Synthase (CerS) Schlank regulates both ceramide synthesis and fat metabolism. Schlank contains a catalytic lag1p motif and, like many CerS in other species, a homeodomain of unknown function. Here, we show that the Drosophila CerS Schlank is imported into the nucleus and requires two nuclear localization signals (NLSs) within its homeodomain and functional Importin-β import machinery. Expression of Schlank variants containing the homeodomain without functional lag1p motif rescued the fat metabolism phenotype of schlank mutants whereas a variant with a mutated NLS site did not rescue. Thus, the homeodomain of Schlank is involved in the regulation of lipid metabolism independent of the catalytic lag1p motif.

Citation

Voelzmann, A., Wulf, A. L., Eckardt, F., Thielisch, M., Brondolin, M., Pesch, Y. Y., Sociale, M., Bauer, R., & Hoch, M. (2016). Nuclear Drosophila CerS Schlank regulates lipid homeostasis via the homeodomain, independent of the lag1p motif. FEBS Letters, 590(7), 971-981. https://doi.org/10.1002/1873-3468.12125

Journal Article Type Article
Acceptance Date Mar 3, 2016
Online Publication Date Mar 7, 2016
Publication Date Apr 1, 2016
Deposit Date Feb 8, 2024
Journal FEBS Letters
Print ISSN 0014-5793
Electronic ISSN 1873-3468
Publisher Wiley
Peer Reviewed Peer Reviewed
Volume 590
Issue 7
Pages 971-981
DOI https://doi.org/10.1002/1873-3468.12125
Keywords Ceramide synthase; Drosophila; Homeodomain; Importin; Lipid homeostasis; Nuclear function
Public URL https://hull-repository.worktribe.com/output/4371694