Nuclear Drosophila CerS Schlank regulates lipid homeostasis via the homeodomain, independent of the lag1p motif

Voelzmann, André; Wulf, Anna Lena; Eckardt, Franka; Thielisch, Melanie; Brondolin, Mirco; Pesch, Yanina Yasmin; Sociale, Mariangela; Bauer, Reinhard; Hoch, Michael

Abstract

Drosophila Ceramide Synthase (CerS) Schlank regulates both ceramide synthesis and fat metabolism. Schlank contains a catalytic lag1p motif and, like many CerS in other species, a homeodomain of unknown function. Here, we show that the Drosophila CerS Schlank is imported into the nucleus and requires two nuclear localization signals (NLSs) within its homeodomain and functional Importin-β import machinery. Expression of Schlank variants containing the homeodomain without functional lag1p motif rescued the fat metabolism phenotype of schlank mutants whereas a variant with a mutated NLS site did not rescue. Thus, the homeodomain of Schlank is involved in the regulation of lipid metabolism independent of the catalytic lag1p motif.

Citation

Voelzmann, A., Wulf, A. L., Eckardt, F., Thielisch, M., Brondolin, M., Pesch, Y. Y., Sociale, M., Bauer, R., & Hoch, M. (2016). Nuclear Drosophila CerS Schlank regulates lipid homeostasis via the homeodomain, independent of the lag1p motif. FEBS Letters, 590(7), 971-981. https://doi.org/10.1002/1873-3468.12125

• Journal Article Type: Article
• Acceptance Date: Mar 3, 2016
• Online Publication Date: Mar 7, 2016
• Publication Date: Apr 1, 2016
• Deposit Date: Feb 8, 2024
• Journal: FEBS Letters
• Print ISSN: 0014-5793
• Electronic ISSN: 1873-3468
• Publisher: Wiley
• Peer Reviewed: Peer Reviewed
• Volume: 590
• Issue: 7
• Pages: 971-981
• DOI: https://doi.org/10.1002/1873-3468.12125
• Keywords: Ceramide synthase; Drosophila; Homeodomain; Importin; Lipid homeostasis; Nuclear function
• Public URL: https://hull-repository.worktribe.com/output/4371694