The repeatability of the abbreviated (4-h) Oral Fat Tolerance Test and influence of prior acute aerobic exercise

O’Doherty, A. F.; Sathyapalan, T.; Rigby, A. S.; Ingle, L.; Carroll, S.

Abstract

© 2016 The Author(s) Purpose: The Oral Fat Tolerance Test (OFTT) is regarded as a repeatable measure used to assess postprandial triglyceride (TAG) levels, with higher levels observed in cardio-metabolic disorders. Acute aerobic exercise intervention before OFTT reduces the TAG response, but the repeatability of this effect is unknown. The aim of this study was to determine the repeatability of the abbreviated 4-h OFTT with and without immediate prior aerobic exercise. Methods: On four separate days, healthy adult male participants underwent two 4-h OFTT (n = 10) and another two 4-h OFTT with 1-h of standardised moderate intensity aerobic exercise performed immediately before meal ingestion (n = 11). The OFTT meal composition included 75.4 g total fat, 21.7 g carbohydrate and 13.7 g protein. Venous blood was sampled at baseline and hourly up to 4 h after the OFTT meal ingestion, and TAG area under the curve (AUC) was calculated. Results: Nonparametric Bland–Altman analysis of 4-h TAG AUC revealed that 9 of 10 repeat measurements fell within ±15 % of the median TAG AUC for the OFTT. By contrast, two of 11 repeat measurements fell within ±15 % of the median TAG AUC for the OFTT undertaken with 1-h prior aerobic exercise. Conclusions: The 4-h OFTT is a repeatable test of postprandial TAG responses in healthy men. However, aerobic exercise performed immediately before OFTT considerably increases the variability of TAG AUC. These findings have implications for interpretation of research studies investigating exercise intervention performed immediately before OFTT. Future studies should also investigate the repeatability of exercise performed 8–24 h before OFTT.

Citation

O’Doherty, A. F., Sathyapalan, T., Rigby, A. S., Ingle, L., & Carroll, S. (2018). The repeatability of the abbreviated (4-h) Oral Fat Tolerance Test and influence of prior acute aerobic exercise. European Journal of Nutrition, 57(1), 309-318. https://doi.org/10.1007/s00394-016-1320-z

• Journal Article Type: Article
• Acceptance Date: Sep 29, 2016
• Online Publication Date: Oct 14, 2016
• Publication Date: 2018-02
• Deposit Date: Oct 18, 2016
• Publicly Available Date: Oct 18, 2016
• Journal: European journal of nutrition
• Print ISSN: 1436-6207
• Electronic ISSN: 1436-6215
• Publisher: Springer Verlag
• Peer Reviewed: Peer Reviewed
• Volume: 57
• Issue: 1
• Pages: 309-318
• DOI: https://doi.org/10.1007/s00394-016-1320-z
• Keywords: OFTT; Postprandial metabolism; Acute exercise; Lipids; Repeatability
• Public URL: https://hull-repository.worktribe.com/output/444244
• Publisher URL: http://link.springer.com/article/10.1007/s00394-016-1320-z
• Additional Information: This is a description of an article which has been published in: European journal of nutrition, 2016