Hepatocellular adenoma in a European flatfish (Limanda limanda) : genetic alterations in laser-capture microdissected tissue and global transcriptomic approach

Lerebours, Adélaïde; Lyons, Brett P.; Bignell, John P.; Stentiford, Grant D.; Rotchell, Jeanette M.; Lerebours, Adelaide; Chapman, Emma; Rotchell, Jeanette; Bignell, John; Lyons, Brett; Stentiford, Grant

Abstract

Liver tumours in flatfish have been diagnosed using histopathology for decades to monitor the impacts of marine pollution. Here we describe the application of specific gene (retinoblastoma, Rb) profiling in laser capture micro-dissected samples, and a suppression subtractive hybridization (SSH) approach to isolate differentially expressed genes in hepatocellular adenoma (HCA) samples from dab, Limanda limanda. The Rb profiles from apparently normal and HCA micro-dissected samples of fish from the North Sea showed no significant difference, and genotypic heterogeneity within defined histological phenotypes was observed. In the SSH, sequences associated with cell signalling, cell cycle, gene expression regulation, protein transport and protein degradation were isolated. These included up-regulation of arrestin domain containing 3 (arrdc3), Rac-1 and tribbles, and down-regulation of ankyrin repeat/sterile alpha-motif domain-containing protein 1B-like (ANKS1B-like), c-fos, CDKN1B and RhoA-like sequences, previously implicated in mammalian HCA. This study offers new candidates involved in fish liver tumour development.

Citation

Lerebours, A., Lyons, B. P., Bignell, J. P., Stentiford, G. D., Rotchell, J. M., Lerebours, A., Chapman, E., Rotchell, J., Bignell, J., Lyons, B., & Stentiford, G. (2017). Hepatocellular adenoma in a European flatfish (Limanda limanda) : genetic alterations in laser-capture microdissected tissue and global transcriptomic approach. Marine pollution bulletin, 119(2), 120-127. https://doi.org/10.1016/j.marpolbul.2017.04.052

• Acceptance Date: Apr 26, 2017
• Online Publication Date: May 3, 2017
• Publication Date: Jun 30, 2017
• Deposit Date: Apr 27, 2017
• Publicly Available Date: May 11, 2018
• Journal: Marine pollution bulletin
• Print ISSN: 0025-326X
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 119
• Issue: 2
• Pages: 120-127
• DOI: https://doi.org/10.1016/j.marpolbul.2017.04.052
• Keywords: Fish, Liver cancer, Retinoblastoma gene, Genetic changes, Contaminants
• Public URL: https://hull-repository.worktribe.com/output/450909
• Publisher URL: http://www.sciencedirect.com/science/article/pii/S0025326X17303594
• Additional Information: This article is maintained by: Elsevier; Article Title: Hepatocellular adenoma in a European flatfish (Limanda limanda): Genetic alterations in laser-capture micro-dissected tissue and global transcriptomic approach; Journal Title: Marine Pollution Bulletin; CrossRef DOI link to publisher maintained version: http://dx.doi.org/10.1016/j.marpolbul.2017.04.052; Content Type: article; Copyright: © 2017 Elsevier Ltd. All rights reserved.
• Contract Date: Apr 27, 2017