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Hepatocellular adenoma in a European flatfish (Limanda limanda) : genetic alterations in laser-capture microdissected tissue and global transcriptomic approach

Lyons, Brett P.; Lerebours, Adélaïde; Bignell, John P.; Stentiford, Grant D.; Rotchell, Jeanette M.; Lerebours, Adelaide; Chapman, Emma; Rotchell, Jeanette; Bignell, John; Lyons, Brett; Stentiford, Grant

Authors

Brett P. Lyons

Adélaïde Lerebours

John P. Bignell

Grant D. Stentiford

Jeanette M. Rotchell

Adelaide Lerebours

Emma Chapman

John Bignell

Brett Lyons

Grant Stentiford



Abstract

Liver tumours in flatfish have been diagnosed using histopathology for decades to monitor the impacts of marine pollution. Here we describe the application of specific gene (retinoblastoma, Rb) profiling in laser capture micro-dissected samples, and a suppression subtractive hybridization (SSH) approach to isolate differentially expressed genes in hepatocellular adenoma (HCA) samples from dab, Limanda limanda. The Rb profiles from apparently normal and HCA micro-dissected samples of fish from the North Sea showed no significant difference, and genotypic heterogeneity within defined histological phenotypes was observed. In the SSH, sequences associated with cell signalling, cell cycle, gene expression regulation, protein transport and protein degradation were isolated. These included up-regulation of arrestin domain containing 3 (arrdc3), Rac-1 and tribbles, and down-regulation of ankyrin repeat/sterile alpha-motif domain-containing protein 1B-like (ANKS1B-like), c-fos, CDKN1B and RhoA-like sequences, previously implicated in mammalian HCA. This study offers new candidates involved in fish liver tumour development.

Citation

Lerebours, A., Lyons, B. P., Bignell, J. P., Stentiford, G. D., Rotchell, J. M., Lerebours, A., …Stentiford, G. (2017). Hepatocellular adenoma in a European flatfish (Limanda limanda) : genetic alterations in laser-capture microdissected tissue and global transcriptomic approach. Marine pollution bulletin, 119(2), 120-127. https://doi.org/10.1016/j.marpolbul.2017.04.052

Acceptance Date Apr 26, 2017
Online Publication Date May 3, 2017
Publication Date Jun 30, 2017
Deposit Date Apr 27, 2017
Publicly Available Date May 11, 2018
Journal Marine pollution bulletin
Print ISSN 0025-326X
Electronic ISSN 1879-3363
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 119
Issue 2
Pages 120-127
DOI https://doi.org/10.1016/j.marpolbul.2017.04.052
Keywords Fish, Liver cancer, Retinoblastoma gene, Genetic changes, Contaminants
Public URL https://hull-repository.worktribe.com/output/450909
Publisher URL http://www.sciencedirect.com/science/article/pii/S0025326X17303594
Additional Information This article is maintained by: Elsevier; Article Title: Hepatocellular adenoma in a European flatfish (Limanda limanda): Genetic alterations in laser-capture micro-dissected tissue and global transcriptomic approach; Journal Title: Marine Pollution Bulletin; CrossRef DOI link to publisher maintained version: http://dx.doi.org/10.1016/j.marpolbul.2017.04.052; Content Type: article; Copyright: © 2017 Elsevier Ltd. All rights reserved.

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