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Genistein crosses the bioartificial oviduct and alters secretion composition

Simintiras, Constantine A.; Sturmey, Roger G.

Authors

Constantine A. Simintiras



Abstract

The dietary derived isoflavone and oestrogen analogue, genistein, is known to perturb fundamental reproductive events such as implantation and embryo cleavage. However the question of whether genistein is able to traverse the oviduct epithelial monolayer and impact oviduct fluid secretion remains unclear. This study tests these research questions using a bioartificial oviduct to show that genistein permeates the oviduct lumen in vitro with a biphasic (burst and plateau) kinetic profile, faster than spontaneous diffusion, and alters the amino acid composition of in vitro derived oviduct fluid (ivDOF) but not as an oestrogen analogue. In addition to offering insights into the potential mechanisms of these findings, this manuscript demonstrates the potential to use the bioartificial oviduct model to characterise the transport or barrier properties of the oviduct towards a range of circulating xenobiotics.

Citation

Simintiras, C. A., & Sturmey, R. G. (2017). Genistein crosses the bioartificial oviduct and alters secretion composition. Reproductive Toxicology, 71, (63-70). doi:10.1016/j.reprotox.2017.04.010. ISSN 0890-6238

Journal Article Type Article
Acceptance Date Apr 27, 2017
Online Publication Date Apr 28, 2017
Publication Date 2017-08
Deposit Date May 5, 2017
Publicly Available Date May 1, 2018
Journal Reproductive toxicology
Print ISSN 0890-6238
Electronic ISSN 1873-1708
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 71
Pages 63-70
DOI https://doi.org/10.1016/j.reprotox.2017.04.010
Keywords Oviduct; Fallopian tube; Genistein; Phytoestrogen; Isoflavone; In vitro derived oviduct fluid
Public URL https://hull-repository.worktribe.com/output/451030
Publisher URL http://www.sciencedirect.com/science/article/pii/S0890623816304725
Copyright Statement ©2018, Elsevier. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
Additional Information This is the accepted manuscript of an article published in Reproductive toxicology, 2017. The version of record is available at the DOI link in this record.

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