Genistein crosses the bioartificial oviduct and alters secretion composition

Simintiras, Constantine A.; Sturmey, Roger G.

Abstract

The dietary derived isoflavone and oestrogen analogue, genistein, is known to perturb fundamental reproductive events such as implantation and embryo cleavage. However the question of whether genistein is able to traverse the oviduct epithelial monolayer and impact oviduct fluid secretion remains unclear. This study tests these research questions using a bioartificial oviduct to show that genistein permeates the oviduct lumen in vitro with a biphasic (burst and plateau) kinetic profile, faster than spontaneous diffusion, and alters the amino acid composition of in vitro derived oviduct fluid (ivDOF) but not as an oestrogen analogue. In addition to offering insights into the potential mechanisms of these findings, this manuscript demonstrates the potential to use the bioartificial oviduct model to characterise the transport or barrier properties of the oviduct towards a range of circulating xenobiotics.

Citation

Simintiras, C. A., & Sturmey, R. G. (2017). Genistein crosses the bioartificial oviduct and alters secretion composition. Reproductive Toxicology, 71, 63-70. https://doi.org/10.1016/j.reprotox.2017.04.010

• Journal Article Type: Article
• Acceptance Date: Apr 27, 2017
• Online Publication Date: Apr 28, 2017
• Publication Date: 2017-08
• Deposit Date: May 5, 2017
• Publicly Available Date: May 1, 2018
• Journal: Reproductive toxicology
• Print ISSN: 0890-6238
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 71
• Pages: 63-70
• DOI: https://doi.org/10.1016/j.reprotox.2017.04.010
• Keywords: Oviduct; Fallopian tube; Genistein; Phytoestrogen; Isoflavone; In vitro derived oviduct fluid
• Public URL: https://hull-repository.worktribe.com/output/451030
• Publisher URL: http://www.sciencedirect.com/science/article/pii/S0890623816304725
• Additional Information: This is the accepted manuscript of an article published in Reproductive toxicology, 2017. The version of record is available at the DOI link in this record.
• Contract Date: May 1, 2018