University of Hull logo

A quinoline-based fluorometric and colorimetric dual-modal pH probe and its application in bioimaging

Zhu, Qin; Li, Zhao; Mu, Lan; Zeng, Xi; Redshaw, Carl; Wei, Gang


Zhao Li

Lan Mu

Gang Wei

Xi Zeng

Qin Zhu


The compound (E)-8-hydroxyl-2-[(E)-2-(2, 4-dihydroxyphenyl)vinyl]-quinoline (1) has been developed as a fluorometric and colorimetric dual-modal probe for pH detection in solution and in vivo. Remarkable changes in the fluorescence intensity with large Stokes shifts and colorimetric responses were observed as a function of pH. The sensing mechanisms involving protonation and deprotonation processes over the acidic and alkaline pH ranges were confirmed by 1H NMR and IR spectroscopic analysis. Furthermore, the application of probe 1 for the imaging of live PC3 cells was successfully achieved. Test strips based on probe 1 were fabricated, and were found to act as a convenient and efficient pH test kits.

Journal Article Type Article
Publication Date Jan 5, 2018
Journal Spectrochimica acta part A : molecular and biomolecular spectroscopy
Print ISSN 1386-1425
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 188
Pages 230-236
Keywords Quinoline; pH probe; Fluorometric; Colorimetric; Bioimaging
Publisher URL
Copyright Statement ©2018, Elsevier. This manuscript version is made available under the CC-BY-NC-ND 4.0 license
Additional Information This is the accepted manuscript of an article published in Spectrochimica acta part A : molecular and biomolecular spectroscopy, 2018. The version of record is available at the DOI link in this record.


Downloadable Citations