A quinoline-based fluorometric and colorimetric dual-modal pH probe and its application in bioimaging

Zhu, Qin; Li, Zhao; Mu, Lan; Zeng, Xi; Redshaw, Carl; Wei, Gang

Abstract

The compound (E)-8-hydroxyl-2-[(E)-2-(2, 4-dihydroxyphenyl)vinyl]-quinoline (1) has been developed as a fluorometric and colorimetric dual-modal probe for pH detection in solution and in vivo. Remarkable changes in the fluorescence intensity with large Stokes shifts and colorimetric responses were observed as a function of pH. The sensing mechanisms involving protonation and deprotonation processes over the acidic and alkaline pH ranges were confirmed by 1H NMR and IR spectroscopic analysis. Furthermore, the application of probe 1 for the imaging of live PC3 cells was successfully achieved. Test strips based on probe 1 were fabricated, and were found to act as a convenient and efficient pH test kits.

Citation

Zhu, Q., Li, Z., Mu, L., Zeng, X., Redshaw, C., & Wei, G. (2018). A quinoline-based fluorometric and colorimetric dual-modal pH probe and its application in bioimaging. Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy, 188, 230-236. https://doi.org/10.1016/j.saa.2017.06.071

• Journal Article Type: Article
• Acceptance Date: Jun 30, 2017
• Online Publication Date: Jul 2, 2017
• Publication Date: Jan 5, 2018
• Deposit Date: Aug 17, 2017
• Publicly Available Date: Jul 9, 2018
• Journal: Spectrochimica acta part A : molecular and biomolecular spectroscopy
• Print ISSN: 1386-1425
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 188
• Pages: 230-236
• DOI: https://doi.org/10.1016/j.saa.2017.06.071
• Keywords: Quinoline; pH probe; Fluorometric; Colorimetric; Bioimaging
• Public URL: https://hull-repository.worktribe.com/output/454176
• Publisher URL: http://www.sciencedirect.com/science/article/pii/S1386142517305486?via%3Dihub
• Additional Information: This is the accepted manuscript of an article published in Spectrochimica acta part A : molecular and biomolecular spectroscopy, 2018. The version of record is available at the DOI link in this record.
• Contract Date: Aug 17, 2017