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Chemosensor for micro to nano-molar detection of Ag+ and Hg2+ ions in pure aqueous media and its applications in cell imaging

Nandre, Jitendra P.; Patil, Samadhan R.; Sahoo, Suban K.; Pradeep, Chullikkattil P.; Churakov, Andrei; Yu, Fabiao; Chen, Lingxin; Redshaw, Carl; Patil, Ashok A.; Patil, Umesh D.

Authors

Lingxin Chen

Andrei Churakov

Fabiao Yu

Jitendra P. Nandre

Samadhan R. Patil

Suban K. Sahoo

Chullikkattil P. Pradeep

Ashok A. Patil

Umesh D. Patil

Abstract

The pyridine substituted thiourea derivative PTB-1 was synthesized and characterized by spectroscopic techniques as well as by single crystal X-ray crystallography. The metal ion sensing ability of PTB-1 was explored by various experimental (naked-eye, UV-Vis, fluorescence, mass spectrometry and 1H NMR spectroscopy) and theoretical (B3LYP/6-31G**/LANL2DZ) methods. PTB-1 exhibited a highly selective naked-eye detectable color change from colorless to dark brown and UV-Vis spectral changes for the detection of Ag+ with a detection limit of 3.67 µM in aqueous medium. The detection of Ag+ ions was achieved by test paper strip and supported silica methods. In contrast, PTB-1 exhibited a 23-fold enhanced emission at 420 nm in the presence of Hg2+ ions with a nano-molar detection limit of 0.69 nM. Finally, the sensor PTB-1 was applied successfully for the intracellular detection of Hg2+ ions in a HepG2 liver cell line, which was monitored by use of confocal imaging techniques.

Journal Article Type Article
Publication Date Jan 1, 2017
Journal Dalton transactions
Print ISSN 1477-9226
Electronic ISSN 1477-9234
Publisher Royal Society of Chemistry
Peer Reviewed Peer Reviewed
Volume 46
Issue 41
Pages 14201-14209
DOI https://doi.org/10.1039/C7DT02524F
Keywords Inorganic Chemistry
Publisher URL http://pubs.rsc.org/en/Content/ArticleLanding/2017/DT/C7DT02524F#!divAbstract
Additional Information : This document is Similarity Check deposited; : Supplementary Information; : Crystal Structure Data; : Suban K. Sahoo (ORCID); : Chullikkattil P. Pradeep (ORCID); : Lingxin Chen (ORCID); : Carl Redshaw (ORCID); : Umesh D. Patil (ORCID); : The Royal Society of Chemistry has an exclusive publication licence for this journal; OPEN ACCESS: This article is freely available. This article is licensed under a Creative Commons Attribution Non Commercial 3.0 Unported Licence (CC BY-NC 3.0); : Single-blind; : Received 12 July 2017; Accepted 19 September 2017; Accepted Manuscript published 19 September 2017; Advance Article published 9 October 2017; Version of Record published 24 October 2017

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This work is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License.



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