Chemosensor for micro to nano-molar detection of Ag+ and Hg2+ ions in pure aqueous media and its applications in cell imaging

Nandre, Jitendra P.; Patil, Samadhan R.; Sahoo, Suban K.; Pradeep, Chullikkattil P.; Churakov, Andrei; Yu, Fabiao; Chen, Lingxin; Redshaw, Carl; Patil, Ashok A.; Patil, Umesh D.

Abstract

The pyridine substituted thiourea derivative PTB-1 was synthesized and characterized by spectroscopic techniques as well as by single crystal X-ray crystallography. The metal ion sensing ability of PTB-1 was explored by various experimental (naked-eye, UV-Vis, fluorescence, mass spectrometry and 1H NMR spectroscopy) and theoretical (B3LYP/6-31G**/LANL2DZ) methods. PTB-1 exhibited a highly selective naked-eye detectable color change from colorless to dark brown and UV-Vis spectral changes for the detection of Ag+ with a detection limit of 3.67 µM in aqueous medium. The detection of Ag+ ions was achieved by test paper strip and supported silica methods. In contrast, PTB-1 exhibited a 23-fold enhanced emission at 420 nm in the presence of Hg2+ ions with a nano-molar detection limit of 0.69 nM. Finally, the sensor PTB-1 was applied successfully for the intracellular detection of Hg2+ ions in a HepG2 liver cell line, which was monitored by use of confocal imaging techniques.

Citation

Nandre, J. P., Patil, S. R., Sahoo, S. K., Pradeep, C. P., Churakov, A., Yu, F., Chen, L., Redshaw, C., Patil, A. A., & Patil, U. D. (2017). Chemosensor for micro to nano-molar detection of Ag+ and Hg2+ ions in pure aqueous media and its applications in cell imaging. Dalton Transactions : an international journal of inorganic chemistry, 46(41), 14201-14209. https://doi.org/10.1039/C7DT02524F

• Journal Article Type: Article
• Acceptance Date: Sep 17, 2017
• Publication Date: Jan 1, 2017
• Deposit Date: Sep 27, 2017
• Publicly Available Date: Sep 19, 2018
• Journal: Dalton transactions
• Print ISSN: 1477-9226
• Electronic ISSN: 1477-9234
• Publisher: Royal Society of Chemistry
• Peer Reviewed: Peer Reviewed
• Volume: 46
• Issue: 41
• Pages: 14201-14209
• DOI: https://doi.org/10.1039/C7DT02524F
• Keywords: Inorganic Chemistry
• Public URL: https://hull-repository.worktribe.com/output/455210
• Publisher URL: http://pubs.rsc.org/en/Content/ArticleLanding/2017/DT/C7DT02524F#!divAbstract
• Contract Date: Sep 27, 2017