Comprehensive characterization of myeloid cells during wound healing in healthy and healing-impaired diabetic mice

Joshi, Natasha; Pohlmeier, Lea; Ben-Yehuda Greenwald, Maya; Haertel, Eric; Hiebert, Paul; Kopf, Manfred; Werner, Sabine

Abstract

Wound healing involves the concerted action of various lymphoid and in particular myeloid cell populations. To characterize and quantitate different types of myeloid cells and to obtain information on their kinetics during wound healing, we performed multiparametric flow cytometry analysis. In healthy mice, neutrophil numbers increased early after injury and returned to near basal levels after completion of healing. Macrophages, monocyte-derived dendritic cells (DCs), and eosinophils were abundant throughout the healing phase, in particular in early wounds, and Langerhans cells increased after wounding and remained elevated after epithelial closure. Major differences in healing-impaired diabetic mice were a much higher percentage of immune cells in late wounds, mainly as a result of neutrophil, macrophage, and monocyte persistence; reduced numbers and percentages of macrophages and monocyte-derived DCs in early wounds; and of Langerhans cells, conventional DCs, and eosinophils throughout the healing process. Finally, unbiased cluster analysis (PhenoGraph) identified a large number of different clusters of myeloid cells in skin wounds. These results provide insight into myeloid cell diversity and dynamics during wound repair and highlight the abnormal inflammatory response associated with impaired healing.

Citation

Joshi, N., Pohlmeier, L., Ben-Yehuda Greenwald, M., Haertel, E., Hiebert, P., Kopf, M., & Werner, S. (2020). Comprehensive characterization of myeloid cells during wound healing in healthy and healing-impaired diabetic mice. European journal of immunology, 50(9), 1335-1349. https://doi.org/10.1002/eji.201948438

• Journal Article Type: Article
• Acceptance Date: Apr 16, 2020
• Online Publication Date: Apr 19, 2020
• Publication Date: Sep 1, 2020
• Deposit Date: Feb 20, 2024
• Publicly Available Date: Feb 22, 2024
• Journal: European Journal of Immunology
• Print ISSN: 0014-2980
• Electronic ISSN: 1521-4141
• Publisher: Wiley
• Peer Reviewed: Peer Reviewed
• Volume: 50
• Issue: 9
• Pages: 1335-1349
• DOI: https://doi.org/10.1002/eji.201948438
• Keywords: Inflammation; Myeloid cell; Skin; Tissue repair; Wound healing
• Public URL: https://hull-repository.worktribe.com/output/4554043