Environmental factors influence cross-talk between a heat shock protein and an oxidative stress protein modification in the lizard Gallotia galloti

Gilbert, Edward; Žagar, Anamarija; López-Darias, Marta; Megía-Palma, Rodrigo; Lister, Karen A.; Jones, Max Dolton; Carretero, Miguel A.; Serén, Nina; Beltran-Alvarez, Pedro; Wollenberg Valero, Katharina C.

Abstract

Better understanding how organisms respond to their abiotic environment, especially at the biochemical level, is critical in predicting population trajectories under climate change. In this study, we measured constitutive stress biomarkers and protein post-translational modifications associated with oxidative stress in Gallotia galloti, an insular lizard species inhabiting highly heterogeneous environments on Tenerife. Tenerife is a small volcanic island in a relatively isolated archipelago off the West coast of Africa. We found that expression of GRP94, a molecular chaperone protein, and levels of protein carbonylation, a marker of cellular stress, change across different environments, depending on solar radiation-related variables and topology. Here, we report in a wild animal population, cross-talk between the baseline levels of the heat shock protein-like GRP94 and oxidative damage (protein carbonylation), which are influenced by a range of available temperatures, quantified through modelled operative temperature. This suggests a dynamic trade-off between cellular homeostasis and oxidative damage in lizards adapted to this thermally and topologically heterogeneous environment.

Citation

Gilbert, E., Žagar, A., López-Darias, M., Megía-Palma, R., Lister, K. A., Jones, M. D., …Wollenberg Valero, K. C. (2024). Environmental factors influence cross-talk between a heat shock protein and an oxidative stress protein modification in the lizard Gallotia galloti. PLoS ONE, 19(3 March), Article e0300111. https://doi.org/10.1371/journal.pone.0300111

• Journal Article Type: Article
• Acceptance Date: Feb 21, 2024
• Online Publication Date: Mar 12, 2024
• Publication Date: Mar 1, 2024
• Deposit Date: Mar 6, 2024
• Publicly Available Date: Mar 15, 2024
• Journal: PLoS ONE
• Print ISSN: 1932-6203
• Electronic ISSN: 1932-6203
• Publisher: Public Library of Science
• Peer Reviewed: Peer Reviewed
• Volume: 19
• Issue: 3 March
• Article Number: e0300111
• DOI: https://doi.org/10.1371/journal.pone.0300111
• Public URL: https://hull-repository.worktribe.com/output/4574535