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Cell sorting by endocytotic capacity in a microfluidic magnetophoresis device

Robert, Damien; Pamme, Nicole; Conjeaud, Hélène; Gazeau, Florence; Iles, Alexander; Wilhelm, Claire


Damien Robert

Nicole Pamme

Hélène Conjeaud

Florence Gazeau

Alexander Iles

Claire Wilhelm


Magnetically labelled cells are finding a wealth of applications for in vitro analysis as well as in vivo treatments. Sorting of cells into subpopulations based on their magnetite loading is an important step in such procedures. Here, we study the sorting of monocytes and macrophages which internalise nanoparticles to different extents based on their endocytotic capacity. Macrophages featured a high endocytotic activity and were found to internalise between 4 and 60 pg of iron per cell. They were successfully sorted into five subpopulations of narrow iron loading distributions via on-chip free-flow magnetophoresis, thus demonstrating the potential of sorting of relatively similarly loaded cells. Monocytes featured a low endocytotic capacity and took on 1 to 4 pg of iron per cell. Mixtures of monocytes and macrophages were successfully sorted within the free-flow magnetophoresis chip and good purity (> 88%), efficacy (> 60%) and throughput (from 10 to 100 cells s(-1)) could be achieved. The introduced method constitutes a viable tool for studies of endocytotic capacity and sorting/selection of cells based on this functionality.


Robert, D., Pamme, N., Conjeaud, H., Gazeau, F., Iles, A., & Wilhelm, C. (2011). Cell sorting by endocytotic capacity in a microfluidic magnetophoresis device. Lab on a chip, 11(11), 1902-1910.

Journal Article Type Article
Acceptance Date Mar 25, 2011
Online Publication Date Apr 21, 2011
Publication Date Jun 7, 2011
Deposit Date Nov 13, 2014
Journal Lab On A Chip
Print ISSN 1473-0197
Electronic ISSN 1473-0189
Publisher Royal Society of Chemistry
Peer Reviewed Peer Reviewed
Volume 11
Issue 11
Pages 1902-1910
Keywords Iron-oxide; Nanoparticles; Magnetically labeled cells
Public URL
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