Design and evaluation of synthetic silica-based monolithic materials in shrinkable tube for efficient protein extraction

Alzahrani, Eman; Welham, Kevin

Abstract

Sample pretreatment is a required step in proteomics in order to remove interferences and preconcentrate the samples. Much research in recent years has focused on porous monolithic materials since they are highly permeable to liquid flow and show high mass transport compared with more common packed beds. These features are due to the micro-structure within the monolithic silica column which contains both macropores that reduce the back pressure, and mesopores that give good interaction with analytes. The aim of this work was to fabricate a continuous porous silica monolithic rod inside a heat shrinkable tube and to compare this with the same material whose surface has been modified with a C(18) phase, in order to use them for preconcentration/extraction of proteins. The performance of the silica-based monolithic rod was evaluated using eight proteins; insulin, cytochrome C, lysozyme, myoglobin, β-lactoglobulin, ovalbumin, hemoglobin, and bovine serum albumin at a concentration of 60 μM. The results show that recovery of the proteins was achieved by both columns with variable yields; however, the C(18) modified silica monolith gave higher recoveries (92.7 to 109.7%) than the non-modified silica monolith (25.5 to 97.9%). Both silica monoliths can be used with very low back pressure indicating a promising approach for future fabrication of the silica monolith inside a microfluidic device for the extraction of proteins from biological media.

Citation

Alzahrani, E., & Welham, K. (2011). Design and evaluation of synthetic silica-based monolithic materials in shrinkable tube for efficient protein extraction. Analyst, 136(20), 4321-4327. https://doi.org/10.1039/c1an15447h

• Journal Article Type: Article
• Acceptance Date: Aug 4, 2011
• Online Publication Date: Aug 24, 2011
• Publication Date: Oct 21, 2011
• Deposit Date: Nov 13, 2014
• Publicly Available Date: Nov 13, 2014
• Journal: Analyst
• Print ISSN: 0003-2654
• Publisher: Royal Society of Chemistry
• Peer Reviewed: Peer Reviewed
• Volume: 136
• Issue: 20
• Pages: 4321-4327
• DOI: https://doi.org/10.1039/c1an15447h
• Public URL: https://hull-repository.worktribe.com/output/463544
• Publisher URL: http://pubs.rsc.org/en/Content/ArticleLanding/2011/AN/c1an15447h#!divAbstract
• Additional Information: Authors' accepted manuscript of article published in: The analyst, 2011, v.136, issue 20
• Contract Date: Nov 13, 2014