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Design and evaluation of synthetic silica-based monolithic materials in shrinkable tube for efficient protein extraction

Alzahrani, Eman; Welham, Kevin

Authors

Eman Alzahrani

Kevin Welham



Abstract

Sample pretreatment is a required step in proteomics in order to remove interferences and preconcentrate the samples. Much research in recent years has focused on porous monolithic materials since they are highly permeable to liquid flow and show high mass transport compared with more common packed beds. These features are due to the micro-structure within the monolithic silica column which contains both macropores that reduce the back pressure, and mesopores that give good interaction with analytes. The aim of this work was to fabricate a continuous porous silica monolithic rod inside a heat shrinkable tube and to compare this with the same material whose surface has been modified with a C(18) phase, in order to use them for preconcentration/extraction of proteins. The performance of the silica-based monolithic rod was evaluated using eight proteins; insulin, cytochrome C, lysozyme, myoglobin, β-lactoglobulin, ovalbumin, hemoglobin, and bovine serum albumin at a concentration of 60 μM. The results show that recovery of the proteins was achieved by both columns with variable yields; however, the C(18) modified silica monolith gave higher recoveries (92.7 to 109.7%) than the non-modified silica monolith (25.5 to 97.9%). Both silica monoliths can be used with very low back pressure indicating a promising approach for future fabrication of the silica monolith inside a microfluidic device for the extraction of proteins from biological media.

Citation

Alzahrani, E., & Welham, K. (2011). Design and evaluation of synthetic silica-based monolithic materials in shrinkable tube for efficient protein extraction. Analyst, 136(20), 4321-4327. https://doi.org/10.1039/c1an15447h

Journal Article Type Article
Acceptance Date Aug 4, 2011
Online Publication Date Aug 24, 2011
Publication Date Oct 21, 2011
Deposit Date Nov 13, 2014
Publicly Available Date Mar 28, 2024
Journal Analyst
Print ISSN 0003-2654
Electronic ISSN 1364-5528
Publisher Royal Society of Chemistry
Peer Reviewed Peer Reviewed
Volume 136
Issue 20
Pages 4321-4327
DOI https://doi.org/10.1039/c1an15447h
Public URL https://hull-repository.worktribe.com/output/463544
Publisher URL http://pubs.rsc.org/en/Content/ArticleLanding/2011/AN/c1an15447h#!divAbstract
Additional Information Authors' accepted manuscript of article published in: The analyst, 2011, v.136, issue 20

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