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Comparing RADseq and microsatellites to infer complex phylogeographic patterns, an empirical perspective in the Crucian carp, Carassius carassius, L.

Jeffries, Daniel L.; Copp, Gordon H.; Lawson Handley, Lori; Olsén, K. Håkan; Sayer, Carl D.; Hänfling, Bernd

Authors

Gordon H. Copp

Dr Bernd Haenfling B.Haenfling@hull.ac.uk
Senior Lecturer/ Chair of the Evolutionary Biology Group (EvoHull)

Lori Lawson Handley

K. Håkan Olsén

Carl D. Sayer

Daniel L. Jeffries

Abstract

The conservation of threatened species must be underpinned by phylogeographic knowledge. This need is epitomized by the freshwater fish Carassius carassius, which is in decline across much of its European range. Restriction site-associated DNA sequencing (RADseq) is increasingly used for such applications; however, RADseq is expensive, and limitations on sample number must be weighed against the benefit of large numbers of markers. This trade-off has previously been examined using simulation studies; however, empirical comparisons between these markers, especially in a phylogeographic context, are lacking. Here, we compare the results from microsatellites and RADseq for the phylogeography of C. carassius to test whether it is more advantageous to genotype fewer markers (microsatellites) in many samples, or many markers (SNPs) in fewer samples. These data sets, along with data from the mitochondrial cytochrome b gene, agree on broad phylogeographic patterns, showing the existence of two previously unidentified C. carassius lineages in Europe: one found throughout northern and central-eastern European drainages and a second almost exclusively confined to the Danubian catchment. These lineages have been isolated for approximately 2.15 M years and should be considered separate conservation units. RADseq recovered finer population structure and stronger patterns of IBD than microsatellites, despite including only 17.6% of samples (38% of populations and 52% of samples per population). RADseq was also used along with approximate Bayesian computation to show that the postglacial colonization routes of C. carassius differ from the general patterns of freshwater fish in Europe, likely as a result of their distinctive ecology.

Journal Article Type Article
Publication Date 2016-07
Journal Molecular ecology
Print ISSN 0962-1083
Electronic ISSN 1365-294X
Publisher Wiley
Peer Reviewed Peer Reviewed
Volume 25
Issue 13
Pages 2997-3018
DOI https://doi.org/10.1111/mec.13613
Keywords Approximate Bayesian computation, Postglacial recolonisation, Landscape genetics, Conservation biology, Study design, Population structure
Publisher URL http://onlinelibrary.wiley.com/doi/10.1111/mec.13613/abstract
Additional Information Authors' accepted manuscript of article published in: Molecular ecology, 2016, v.25, issue 13.

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