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Comparing the effectiveness of polymer debriding devices using a porcine wound biofilm model

Wilkinson, Holly N.; McBain, Andrew J.; Stephenson, Christian; Hardman, Matthew J.

Authors

Holly N. Wilkinson

Andrew J. McBain

Christian Stephenson

Matthew J. Hardman



Abstract

Objective: Debridement to remove necrotic and/or infected tissue and promote active healing remains a cornerstone of contemporary chronic wound management. While there has been a recent shift toward less invasive polymer-based debriding devices, their efficacy requires rigorous evaluation.

Approach: This study was designed to directly compare monofilament debriding devices to traditional gauze using a wounded porcine skin biofilm model with standardized application parameters. Biofilm removal was determined using a surface viability assay, bacterial counts, histological assessment, and scanning electron microscopy (SEM).

Results: Quantitative analysis revealed that monofilament debriding devices outperformed the standard gauze, resulting in up to 100-fold greater reduction in bacterial counts. Interestingly, histological and morphological analyses suggested that debridement not only removed bacteria, but also differentially disrupted the bacterially-derived extracellular polymeric substance. Finally, SEM of post-debridement monofilaments showed structural changes in attached bacteria, implying a negative impact on viability.

Innovation: This is the first study to combine controlled and defined debridement application with a biologically relevant ex vivo biofilm model to directly compare monofilament debriding devices.

Conclusion: These data support the use of monofilament debriding devices for the removal of established wound biofilms and suggest variable efficacy towards biofilms composed of different species of bacteria.

Journal Article Type Article
Publication Date Nov 1, 2016
Journal Advances in Wound Care
Print ISSN 2162-1918
Electronic ISSN 2162-1934
Peer Reviewed Peer Reviewed
Volume 5
Issue 11
Pages 475-485
APA6 Citation Wilkinson, H. N., McBain, A. J., Stephenson, C., & Hardman, M. J. (2016). Comparing the effectiveness of polymer debriding devices using a porcine wound biofilm model. Advances in wound care, 5(11), 475-485. doi:10.1089/wound.2015.0683
DOI https://doi.org/10.1089/wound.2015.0683
Keywords Critical Care and Intensive Care Medicine; Emergency Medicine
Publisher URL http://online.liebertpub.com/doi/10.1089/wound.2015.0683
Related Public URLs https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5105345/
Copyright Statement © Holly N. Wilkinson, et al., 2016; Published by Mary Ann Liebert, Inc. This Open Access article is distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by/
4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited.

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Copyright Statement
© Holly N. Wilkinson, et al., 2016; Published by Mary Ann Liebert, Inc. This Open Access article is distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by/
4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited.



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