Lutz Christian Gerhardt
Cytocompatibility, bioactivity and mechanical strength of calcium phosphate cement reinforced with multi-walled carbon nanotubes and bovine serum albumin
Gerhardt, Lutz Christian; Boccaccini, Aldo R.
Aldo R. Boccaccini
This paper reports on the in vitro cytotoxicity, bioactivity behaviour and mechanical properties of novel injectable calcium phosphate cement filled with hydroxylated multi-walled carbon nanotubes and bovine serum albumin (CPC/MWCNT-OH/BSA). To predict the in vitro bioactivity of the calcium phosphate composites, we investigated apatite formation on CPC/MWCNT-OH/BSA composites after soaking in simulated body fluid (SBF) for up to 28 days. Compressive strength tests, scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD) and cell culture experiments with human CCD-18Co fibroblasts cell lines were performed to evaluate the effect of SBF pre-treatment on the mechanical, structural and biological properties of the CPC/MWCNT-OH/BSA composites. Although apatite formation increased significantly with SBF immersion period, the results showed that all soaked CPC/MWCNT-OH/BSA composites exhibited up to 2.5 times lower compressive strength (13-20 MPa), which were however higher than values reported for the strength of trabecular bone (2-12 MPa). Cell culture experiments showed that low concentrations (6.25 and 12.5 μg/ml) of bio-mineralised CPC/MWCNT-OH/BSA composites led to cell proliferative rather than cytotoxic effects on fibroblasts, evidenced by high cell viabilities (104-113%). The novel CPC/MWCNT-OH/BSA composites presented in this study showed favourable cytocompatible and bioactive behaviour along with high compressive strength (13-32 MPa) and are therefore considered as an attractive bone filling material. © 2012 Elsevier Ltd and Techna Group S.r.l.
Gholami, F., Zein, S. H. S., Gerhardt, L. C., Low, K. L., Tan, S. H., McPhail, D. S., …Boccaccini, A. R. (2013). Cytocompatibility, bioactivity and mechanical strength of calcium phosphate cement reinforced with multi-walled carbon nanotubes and bovine serum albumin. Ceramics international, 39(5), 4975-4983. https://doi.org/10.1016/j.ceramint.2012.11.094
|Journal Article Type||Article|
|Acceptance Date||Nov 28, 2012|
|Online Publication Date||Dec 25, 2012|
|Deposit Date||Jul 7, 2020|
|Peer Reviewed||Peer Reviewed|
|Keywords||Cytotoxicity; In vitro bioactivity; Compressive strength; CPCs|
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