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Lipase immobilised on silica monoliths as continuous-flow microreactors for triglyceride transesterification

Alotaibi, Mohammed; Manayil, Jinesh C.; Greenway, Gillian M.; Haswell, Stephen J.; Kelly, Stephen M.; Lee, Adam F.; Wilson, Karen; Kyriakou, Georgios

Authors

Mohammed Alotaibi

Jinesh C. Manayil

Gillian M. Greenway

Stephen J. Haswell

Adam F. Lee

Karen Wilson

Georgios Kyriakou



Abstract

Lipase immobilised on silica monoliths has been prepared and applied as biocatalytic continuous-flow microreactors for the transesterification of tributyrin as a model bio-oil component. Candida antarctica lipase was trapped within the pores of silica monoliths, and its successful immobilisation was demonstrated by the hydrolysis of 4-nitrophenyl butyrate to 4-nitrophenol. Lipase immobilised on silica monoliths was active for the transesterification of tributyrin at ambient temperature, with reactivity as a function of the methanol : tributyrin ratio, flow rate, temperature, and textural properties. Monoliths with a high surface area and large meso- and macropore channels enhanced the transesterification activity through improved molecule diffusion. The optimum immobilised lipase microreactor exhibited almost quantitative ester production for >100 h at 30 °C without deactivation.

Journal Article Type Article
Publication Date Dec 18, 2017
Journal Reaction Chemistry & Engineering
Print ISSN 2058-9883
Publisher Royal Society of Chemistry
Peer Reviewed Peer Reviewed
Volume 3
Issue 1
Pages 68-74
APA6 Citation Alotaibi, M., Manayil, J. C., Greenway, G. M., Haswell, S. J., Kelly, S. M., Lee, A. F., …Kyriakou, G. (2017). Lipase immobilised on silica monoliths as continuous-flow microreactors for triglyceride transesterification. Reaction Chemistry and Engineering, 3(1), 68-74. https://doi.org/10.1039/c7re00162b
DOI https://doi.org/10.1039/c7re00162b
Publisher URL http://pubs.rsc.org/en/Content/ArticleLanding/2018/RE/C7RE00162B#!divAbstract
Additional Information : This document is Similarity Check deposited; : Supplementary Information; : Jinesh C. Manayil (ORCID); : Stephen M. Kelly (ORCID); : Adam F. Lee (ORCID); : Adam F. Lee (ResearcherID); : Karen Wilson (ORCID); : Karen Wilson (ResearcherID); : Georgios Kyriakou (ORCID); : Single-blind; : Received 2 October 2017; Accepted 18 December 2017; Accepted Manuscript published 18 December 2017; Advance Article published 5 January 2018; Version of Record published 6 February 2018

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