Lipase immobilised on silica monoliths as continuous-flow microreactors for triglyceride transesterification

Alotaibi, Mohammed; Manayil, Jinesh C.; Greenway, Gillian M.; Haswell, Stephen J.; Kelly, Stephen M.; Lee, Adam F.; Wilson, Karen; Kyriakou, Georgios

Abstract

Lipase immobilised on silica monoliths has been prepared and applied as biocatalytic continuous-flow microreactors for the transesterification of tributyrin as a model bio-oil component. Candida antarctica lipase was trapped within the pores of silica monoliths, and its successful immobilisation was demonstrated by the hydrolysis of 4-nitrophenyl butyrate to 4-nitrophenol. Lipase immobilised on silica monoliths was active for the transesterification of tributyrin at ambient temperature, with reactivity as a function of the methanol : tributyrin ratio, flow rate, temperature, and textural properties. Monoliths with a high surface area and large meso- and macropore channels enhanced the transesterification activity through improved molecule diffusion. The optimum immobilised lipase microreactor exhibited almost quantitative ester production for >100 h at 30 °C without deactivation.

Citation

Alotaibi, M., Manayil, J. C., Greenway, G. M., Haswell, S. J., Kelly, S. M., Lee, A. F., Wilson, K., & Kyriakou, G. (2017). Lipase immobilised on silica monoliths as continuous-flow microreactors for triglyceride transesterification. Reaction Chemistry and Engineering, 3(1), 68-74. https://doi.org/10.1039/c7re00162b

• Journal Article Type: Article
• Acceptance Date: Dec 18, 2017
• Online Publication Date: Dec 18, 2017
• Publication Date: Dec 18, 2017
• Deposit Date: Jan 22, 2018
• Publicly Available Date: Dec 19, 2018
• Journal: Reaction Chemistry & Engineering
• Print ISSN: 2058-9883
• Publisher: Royal Society of Chemistry
• Peer Reviewed: Peer Reviewed
• Volume: 3
• Issue: 1
• Pages: 68-74
• DOI: https://doi.org/10.1039/c7re00162b
• Public URL: https://hull-repository.worktribe.com/output/552168
• Publisher URL: http://pubs.rsc.org/en/Content/ArticleLanding/2018/RE/C7RE00162B#!divAbstract
• Additional Information: : This document is Similarity Check deposited; : Supplementary Information; : Jinesh C. Manayil (ORCID); : Stephen M. Kelly (ORCID); : Adam F. Lee (ORCID); : Adam F. Lee (ResearcherID); : Karen Wilson (ORCID); : Karen Wilson (ResearcherID); : Georgios Kyriakou (ORCID); : Single-blind; : Received 2 October 2017; Accepted 18 December 2017; Accepted Manuscript published 18 December 2017; Advance Article published 5 January 2018; Version of Record published 6 February 2018
• Contract Date: Jan 29, 2018