Simon Scrace
Use of the xCELLigence system for real-time analysis of changes in cellular motility and adhesion in physiological conditions
Scrace, Simon; O'Neill, Eric; Hammond, Ester M.; Pires, Isabel M.
Authors
Eric O'Neill
Ester M. Hammond
Isabel M. Pires
Contributors
Isabel Monteiro dos Santos Pires
Other
Abstract
Investigation of the mechanisms behind the regulation of cellular motility and adhesion is key to understanding metastasis and the biology of tumor spreading. There are many technologies available for these studies, but the majority of them are either dependent on the use of labels or limited to endpoint analysis. The xCELLigence RTCA (real-time cell analysis) provides a platform for label free and operator independent investigation of the migration, invasion and adhesion proprieties of cells in physiologically relevant conditions. The real-time kinetic data acquisition also allows for a more accurate characterization of short-lived cellular events. In this chapter we describe the use of the xCELLigence Real-Time Cell Analyzer to investigate changes in cellular adhesion and motility in real time. © Springer Science+Business Media, LLC 2013.
Citation
Scrace, S., O'Neill, E., Hammond, E. M., & Pires, I. M. (2013). Use of the xCELLigence system for real-time analysis of changes in cellular motility and adhesion in physiological conditions. Methods in molecular biology, 1046, 295-306. https://doi.org/10.1007/978-1-62703-538-5_17
Journal Article Type | Article |
---|---|
Acceptance Date | Mar 1, 2013 |
Online Publication Date | Jun 27, 2013 |
Publication Date | Jan 1, 2013 |
Deposit Date | Mar 21, 2018 |
Print ISSN | 1064-3745 |
Electronic ISSN | 1940-6029 |
Volume | 1046 |
Pages | 295-306 |
Book Title | Adhesion Protein Protocols; Methods in Molecular Biology |
Chapter Number | 17 |
ISBN | 9781627035378 |
DOI | https://doi.org/10.1007/978-1-62703-538-5_17 |
Public URL | https://hull-repository.worktribe.com/output/749216 |
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