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DNA barcoding Madagascar's amphibian fauna

Perl, R.G.Bina; Vences, Miguel; Wollenberg, Katharina C.; Sonet, Gontran; Glaw, Frank; Nagy, Zoltán T.

Authors

R.G.Bina Perl

Miguel Vences

Katharina C. Wollenberg

Gontran Sonet

Frank Glaw

Zoltán T. Nagy



Abstract

We provide a DNA barcoding survey of Malagasy amphibians, including 251 of the 292 nominal species known to date, by complementing previous data with 280 newly determined barcoding sequence fragments of the mitochondrial cytochrome oxidase subunit I (COI) gene. Amplification success for the newly determined sequences was highest (94%) with one set of universal COI primers (dgLCO1490-dgHCO2198) while other primer sets had distinctly lower success rates. By and large, we observed relatively high average interspecific genetic distances of 25-27% within the Mantellidae and Microhylidae, and genetic distances of 13-21% within the Hyperoliidae. Lower values of 6-7% were observed between some sister species in all families, with extreme lows of 0.2-0.3% between a few sister species pairs in microhylids and mantellids for which we postulate mitochondrial introgression or yet unsettled taxonomy. Within-species divergences were relatively high especially in mantellids where they averaged 5.3%, due to the inclusion of numerous deep conspecific lineages (by definition with high divergences to other specimens) in our study. Above this, the degree of polymorphism was difficult to establish owing to limited sampling per population in our assessment. Compared to a previous assessment from 2009 based on 16S rDNA sequences, we identify 14 additional undescribed candidate species and raise the maximum estimate of species in the island’s batrachofauna to well over 500.

Citation

Perl, R., Vences, M., Wollenberg, K. C., Sonet, G., Glaw, F., & Nagy, Z. T. (2014). DNA barcoding Madagascar's amphibian fauna. Amphibia-Reptilia, 35(2), 197-206. https://doi.org/10.1163/15685381-00002942

Journal Article Type Article
Acceptance Date Jan 1, 2014
Online Publication Date Jun 2, 2014
Publication Date Jun 2, 2014
Deposit Date Aug 9, 2018
Publicly Available Date Mar 28, 2024
Journal Amphibia-Reptilia
Print ISSN 0173-5373
Electronic ISSN 1568-5381
Publisher Brill Academic Publishers
Peer Reviewed Peer Reviewed
Volume 35
Issue 2
Pages 197-206
DOI https://doi.org/10.1163/15685381-00002942
Keywords cytochrome c oxidase subunit 1; Anura; Amphibia; Mantellidae; Madagascar; Microhylidae
Public URL https://hull-repository.worktribe.com/output/973885
Publisher URL http://booksandjournals.brillonline.com/content/journals/10.1163/15685381-00002942