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Dr Giordano Pula's Outputs (9)

Oxygen Concentration Plays a Critical Role in Fibrinogen-Mediated Platelet Activation via Inactivation of αIIbβ3 and Modulation of Fibrinogen (2025)
Journal Article
Leonard, S. V. L., Booth, Z., Naylor-Adamson, L., Bibby, L., Wraith, K. S., Pula, G., Arman, M., & Calaminus, S. D. J. (2025). Oxygen Concentration Plays a Critical Role in Fibrinogen-Mediated Platelet Activation via Inactivation of αIIbβ3 and Modulation of Fibrinogen. Biomolecules, 15(4), Article 501. https://doi.org/10.3390/biom15040501

In the vascular system, pathological conditions that cause hypoxia are associated with increased platelet activity and thrombosis. Using a platelet spreading assay, we show that severe hypoxia (i.e., 1%), venous (i.e., 5%), and, surprisingly, arteria... Read More about Oxygen Concentration Plays a Critical Role in Fibrinogen-Mediated Platelet Activation via Inactivation of αIIbβ3 and Modulation of Fibrinogen.

Hemoglobin in the brain frontal lobe tissue of patients with Alzheimer's disease is susceptible to reactive nitrogen species-mediated oxidative damage (2025)
Journal Article
Smallwood, M. J., Alghayth, M. A., Knight, A. R., Tveen-Jensen, K., Pitt, A. R., Spickett, C. M., Llewellyn, D., Pula, G., Wearn, A., Vanhatalo, A., Jones, A. M., Francis, P., Coulthard, E., Kehoe, P. G., & Winyard, P. G. (2025). Hemoglobin in the brain frontal lobe tissue of patients with Alzheimer's disease is susceptible to reactive nitrogen species-mediated oxidative damage. Redox Biology, 82, Article 103612. https://doi.org/10.1016/j.redox.2025.103612

Brain inflammation in Alzheimer's disease (AD) involves reactive nitrogen species (RNS) generation. Protein contents of 3-nitrotyrosine, a product of RNS generation, were assessed in frontal lobe brain homogenates from patients with AD, patients with... Read More about Hemoglobin in the brain frontal lobe tissue of patients with Alzheimer's disease is susceptible to reactive nitrogen species-mediated oxidative damage.

Hyperactivity and Pro-inflammatory Functions of Platelets in Diabetes (2025)
Journal Article
Pula, G., & Greaves, J. (2025). Hyperactivity and Pro-inflammatory Functions of Platelets in Diabetes. Frontiers in Bioscience-Landmark, 30(1), Article 26190. https://doi.org/10.31083/FBL26190

Cardiovascular complications claim the lives of up to 70% of patients with diabetes mellitus (DM). The mechanisms increasing cardiovascular risk in DM remain to be fully understood and successfully addressed. Nonetheless, there is increasing evidence... Read More about Hyperactivity and Pro-inflammatory Functions of Platelets in Diabetes.

Development of New Peptidomimetic NADPH Oxidase Inhibitors with Antithrombotic Properties (2024)
Journal Article
Scalia, E., Chirco, A., Calugi, L., Lenci, E., Pagano, P. J., Pula, G., & Trabocchi, A. (2024). Development of New Peptidomimetic NADPH Oxidase Inhibitors with Antithrombotic Properties. ChemMedChem, 19(19), Article e202400330. https://doi.org/10.1002/cmdc.202400330

The ability of synthetic peptides inhibitors of NOX1 to effectively block the production of ROS by the enzyme was studied with different methodologies. Specifically, taking advantage of our understanding of the active epitope of the regulatory NOX1 s... Read More about Development of New Peptidomimetic NADPH Oxidase Inhibitors with Antithrombotic Properties.

A peptide from the staphylococcal protein Efb binds P-selectin and inhibits the interaction of platelets with leukocytes (2022)
Journal Article
Wallis, S., Wolska, N., Englert, H., Posner, M., Upadhyay, A., Renné, T., Eggleston, I., Bagby, S., & Pula, G. (2022). A peptide from the staphylococcal protein Efb binds P-selectin and inhibits the interaction of platelets with leukocytes. Journal of thrombosis and haemostasis : JTH, 20(3), 729-741. https://doi.org/10.1111/jth.15613

Aims: P-selectin is a key surface adhesion molecule for the interaction of platelets with leukocytes. We have shown previously that the N-terminal domain of Staphylococcus aureus extracellular fibrinogen-binding protein (Efb) binds to P-selectin and... Read More about A peptide from the staphylococcal protein Efb binds P-selectin and inhibits the interaction of platelets with leukocytes.

Identification of the factor XII contact activation site enables sensitive coagulation diagnostics (2021)
Journal Article
Heestermans, M., Naudin, C., Mailer, R. K., Konrath, S., Klaetschke, K., Jämsä, A., Frye, M., Deppermann, C., Pula, G., Kuta, P., Friese, M. A., Gelderblom, M., Sickmann, A., Preston, R. J., Nofer, J. R., Rose-John, S., Butler, L. M., Salomon, O., Stavrou, E. X., & Renné, T. (2021). Identification of the factor XII contact activation site enables sensitive coagulation diagnostics. Nature communications, 12(1), Article 5596. https://doi.org/10.1038/s41467-021-25888-7

Contact activation refers to the process of surface-induced activation of factor XII (FXII), which initiates blood coagulation and is captured by the activated partial thromboplastin time (aPTT) assay. Here, we show the mechanism and diagnostic impli... Read More about Identification of the factor XII contact activation site enables sensitive coagulation diagnostics.

Defective NET clearance contributes to sustained FXII activation in COVID-19-associated pulmonary thrombo-inflammation (2021)
Journal Article
Englert, H., Rangaswamy, C., Deppermann, C., Sperhake, J. P., Krisp, C., Schreier, D., Gordon, E., Konrath, S., Haddad, M., Pula, G., Mailer, R. K., Schlüter, H., Kluge, S., Langer, F., Püschel, K., Panousis, K., Stavrou, E. X., Maas, C., Renné, T., & Frye, M. (2021). Defective NET clearance contributes to sustained FXII activation in COVID-19-associated pulmonary thrombo-inflammation. EBioMedicine, 67, Article 103382. https://doi.org/10.1016/j.ebiom.2021.103382

Background: Coagulopathy and inflammation are hallmarks of Coronavirus disease 2019 (COVID-19) and are associated with increased mortality. Clinical and experimental data have revealed a role for neutrophil extracellular traps (NETs) in COVID-19 dise... Read More about Defective NET clearance contributes to sustained FXII activation in COVID-19-associated pulmonary thrombo-inflammation.

NADPH Oxidases Are Required for Full Platelet Activation In Vitro and Thrombosis In Vivo but Dispensable for Plasma Coagulation and Hemostasis (2020)
Journal Article
Vara, D., Mailer, R. K., Tarafdar, A., Wolska, N., Heestermans, M., Konrath, S., Spaeth, M., Renné, T., Schröder, K., & Pula, G. (2021). NADPH Oxidases Are Required for Full Platelet Activation In Vitro and Thrombosis In Vivo but Dispensable for Plasma Coagulation and Hemostasis. Arteriosclerosis, Thrombosis, and Vascular Biology, 41(2), 683-697. https://doi.org/10.1161/ATVBAHA.120.315565

Objective: Using 3KO (triple NOX [NADPH oxidase] knockout) mice (ie, NOX1-/-/NOX2-/-/NOX4-/-), we aimed to clarify the role of this family of enzymes in the regulation of platelets in vitro and hemostasis in vivo. Approach and Results: 3KO mice displ... Read More about NADPH Oxidases Are Required for Full Platelet Activation In Vitro and Thrombosis In Vivo but Dispensable for Plasma Coagulation and Hemostasis.

Xenotropic and polytropic retrovirus receptor 1 regulates procoagulant platelet polyphosphate (2020)
Journal Article
Mailer, R. K., Allende, M., Heestermans, M., Schweizer, M., Deppermann, C., Frye, M., Pula, G., Odeberg, J., Gelderblom, M., Rose-John, S., Sickmann, A., Blankenberg, S., Huber, T. B., Kubisch, C., Maas, C., Gambaryan, S., Firsov, D., Stavrou, E. X., Butler, L. M., & Renné, T. (2021). Xenotropic and polytropic retrovirus receptor 1 regulates procoagulant platelet polyphosphate. Blood, 137(10), 1392-1405. https://doi.org/10.1182/blood.2019004617

Polyphosphate is a procoagulant inorganic polymer of linear-linked orthophosphate residues. Multiple investigations have established the importance of platelet polyphosphate in blood coagulation; however, the mechanistic details of polyphosphate home... Read More about Xenotropic and polytropic retrovirus receptor 1 regulates procoagulant platelet polyphosphate.