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Platelet releasate promotes skeletal myogenesis by increasing muscle stem cell commitment to differentiation and accelerates muscle regeneration following acute injury

Scully, David; Sfyri, Peggy; Verpoorten, Sandrine; Papadopoulos, Petros; Muñoz-Turrillas, María Carmen; Mitchell, Robert; Aburima, Ahmed; Patel, Ketan; Gutiérrez, Laura; Naseem, Khalid M.; Matsakas, Antonios

Authors

David Scully

Peggy Sfyri

Sandrine Verpoorten

Petros Papadopoulos

María Carmen Muñoz-Turrillas

Robert Mitchell

Ahmed Aburima

Ketan Patel

Laura Gutiérrez

Khalid M. Naseem

Antonios Matsakas



Abstract

Aim

The use of platelets as biomaterials has gained intense research interest. However, the mechanisms regarding platelet‐mediated skeletal myogenesis remain to be established. The aim of this study was to determine the role of platelet releasate in skeletal myogenesis and muscle stem cell fate in vitro and ex vivo respectively.

Methods

We analysed the effect of platelet releasate on proliferation and differentiation of C2C12 myoblasts by means of cell proliferation assays, immunohistochemistry, gene expression and cell bioenergetics. We expanded in vitro findings on single muscle fibres by determining the effect of platelet releasate on murine skeletal muscle stem cells using protein expression profiles for key myogenic regulatory factors.

Results

TRAP6 and collagen used for releasate preparation had a more pronounced effect on myoblast proliferation versus thrombin and sonicated platelets (P less than 0.001). Platelet releasate was detrimental for myoblast fusion and affected differentiation of myoblasts in a temporal manner. Most importantly we show that platelet releasate promotes skeletal myogenesis through the PDGF/VEGF‐Cyclin D1‐MyoD‐Scrib‐Myogenin axis and accelerates skeletal muscle regeneration after acute injury.

Conclusion
This study provides novel mechanistic insights on the role of platelet releasate in skeletal myogenesis and set the physiological basis for exploiting platelets as biomaterials in regenerative medicine.

Journal Article Type Article
Publication Date 2019-03
Journal Acta Physiologica
Print ISSN 1748-1708
Electronic ISSN 1748-1716
Publisher Wiley
Peer Reviewed Peer Reviewed
Volume 225
Issue 3
Article Number e13207
APA6 Citation Scully, D., Sfyri, P., Verpoorten, S., Papadopoulos, P., Muñoz-Turrillas, M. C., Mitchell, R., …Matsakas, A. (2019). Platelet releasate promotes skeletal myogenesis by increasing muscle stem cell commitment to differentiation and accelerates muscle regeneration following acute injury. Acta Physiologica, 225(3), https://doi.org/10.1111/apha.13207
DOI https://doi.org/10.1111/apha.13207
Keywords Growth factors; Platelet releasate; Platelet‐rich plasma; Regeneration; Skeletal muscle; Satellite cells
Publisher URL https://onlinelibrary.wiley.com/doi/abs/10.1111/apha.13207
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