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Optimising platelet secretomes to deliver robust tissue-specific regeneration

Scully, David; Sfyri, Peggy; Wilkinson, Holly N.; Acebes‐Huerta, Andrea; Verpoorten, Sandrine; Muñoz‐Turrillas, María Carmen; Parnell, Andrew; Patel, Ketan; Hardman, Matthew J.; Gutiérrez, Laura; Matsakas, Antonios

Authors

David Scully

Peggy Sfyri

Andrea Acebes‐Huerta

Sandrine Verpoorten

María Carmen Muñoz‐Turrillas

Andrew Parnell

Ketan Patel

Laura Gutiérrez

Antonios Matsakas



Abstract

Promoting cell proliferation is the cornerstone of most tissue regeneration therapies. As platelet-based applications promote cell division and can be customised for tissue-specific efficacy, this makes them strong candidates for developing novel regenerative therapies. Therefore, the aim of this study was to determine if platelet releasate could be optimised to promote cellular proliferation and differentiation of specific tissues. Growth factors in platelet releasate were profiled for physiological and supraphysiological platelet concentrations. We analysed the effect of physiological and supraphysiological releasate on C2C12 skeletal myoblasts, H9C2 rat cardiomyocytes, human dermal fibroblasts (HDF), HaCaT keratinocytes, and chondrocytes. Cellular proliferation and differentiation were assessed through proliferation assays, mRNA, and protein expression. We show that supraphysiological releasate is not simply a concentrated version of physiological releasate. Physiological releasate promoted C2C12, HDF, and chondrocyte proliferation with no effect on H9C2 or HaCaT cells. Supraphysiological releasate induced stronger proliferation in C2C12 and HDF cells compared with physiological releasate. Importantly, supraphysiological releasate induced proliferation of H9C2 cells. The proliferative effects of skeletal and cardiac muscle cells were in part driven by vascular endothelial growth factor alpha. Furthermore, supraphysiological releasate induced differentiation of H9C2 and C2C12, HDF, and keratinocytes. This study provides insights into the ability of releasate to promote muscle, heart, skin, and cartilage cell proliferation and differentiation and highlights the importance of optimising releasate composition for tissue-specific regeneration.

Citation

Scully, D., Sfyri, P., Wilkinson, H. N., Acebes‐Huerta, A., Verpoorten, S., Muñoz‐Turrillas, M. C., …Matsakas, A. (2020). Optimising platelet secretomes to deliver robust tissue-specific regeneration. Journal of tissue engineering and regenerative medicine, 14(1), 82-98. https://doi.org/10.1002/term.2965

Journal Article Type Article
Acceptance Date Sep 9, 2019
Online Publication Date Oct 11, 2019
Publication Date Jan 1, 2020
Deposit Date Oct 14, 2019
Publicly Available Date Oct 12, 2020
Journal Journal of Tissue Engineering and Regenerative Medicine
Print ISSN 1932-6254
Electronic ISSN 1932-7005
Publisher Wiley
Peer Reviewed Peer Reviewed
Volume 14
Issue 1
Pages 82-98
DOI https://doi.org/10.1002/term.2965
Keywords Biomaterial; Cardiomyocyte; Chondrocyte; Fibroblast; Injury; Keratinocyte; Platelet releasate; Regeneration
Public URL https://hull-repository.worktribe.com/output/2909333
Publisher URL https://onlinelibrary.wiley.com/doi/epdf/10.1002/term.2965
Related Public URLs http://centaur.reading.ac.uk/86212/
Additional Information Received: 2019-05-10; Accepted: 2019-09-09; Published: 2019-10-11

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Copyright Statement
©2019 University of Hull





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