Skip to main content

Research Repository

Advanced Search

Effect of high glucose on human alveolar macrophage phenotype and phagocytosis of mycobacteria

Vance, Jesse; Santos, Andres; Sadofsky, Laura; Morice, Alyn; Cervantes, Jorge


Jesse Vance

Andres Santos

Dr Laura Sadofsky
Lecturer in Respiratory Medicine/ Academic lead for postgraduate training in HYMS

Jorge Cervantes


Diabetes mellitus (DBM) reduces immunological activity and increases susceptibility to various infections, including tuberculosis (TB). Human alveolar macrophage (hAM) functions are altered in DBM.

To mimic hyperglycemic conditions in the lung alveolus, we co-cultured a hAM cell line (Daisy cell line) with human umbilical vein endothelial cells for 48 h in the presence of culture media alone, normal glucose (5 mM), and high glucose (22 mM). Using flow cytometry, immunophenotype characterization included cell surface markers CD 11c, CD14, CD16, CD86, CD163, CD169, CD206, CX3CR-1, CSF-1R, and matrix metalloproteinase-9 (MMP9). Phagocytic function was measured by immunofluorescence microscopy at 24 h after inoculation of cells with GFP-expressing Mycobacterium smegmatis.

Direct exposure of AMs to high glucose and exposure in the co-culture system yield different results for the same phenotypic markers. MMP9 expression was increased under both conditions. CD169 and CX3CR1 expressions were decreased when AMs were exposed directly to high glucose but increased under co-culture. Immunofluorescence assay revealed that phagocytosis decreased in AMs when directly exposed to increased glucose levels from 2.5 mM to normal glucose (5 mM), yet AMs under co-culture did not show decreased phagocytosis until concentrations were raised to 25 mM.

Alteration in the expression of certain receptors may contribute to defective sentinel function of AMs, promoting susceptibility to TB in a diabetic host. Variability in cell surface marker expression under direct glucose exposure compared to exposure via co-culture reveals that cell signaling between endothelial cells and AMs may play a crucial role in the phenotypic expression of AMs.


Vance, J., Santos, A., Sadofsky, L., Morice, A., & Cervantes, J. (2019). Effect of high glucose on human alveolar macrophage phenotype and phagocytosis of mycobacteria. Lung, 197(1), 89–94. doi:10.1007/s00408-018-0181-z

Journal Article Type Article
Acceptance Date Nov 19, 2018
Online Publication Date Nov 24, 2018
Publication Date 2019-02
Deposit Date Dec 7, 2018
Publicly Available Date Nov 25, 2019
Journal Lung
Print ISSN 0341-2040
Electronic ISSN 1432-1750
Publisher Springer Verlag
Peer Reviewed Peer Reviewed
Volume 197
Issue 1
Pages 89–94
Keywords Diabetes; Alveolar macrophages; Tuberculosis; Mycobacteria
Public URL
Publisher URL


You might also like

Downloadable Citations