Molecular and culture-based assessment of the microbial diversity of diabetic chronic foot wounds and contralateral skin sites

Oates, Angela; Bowling, Frank L.; Boulton, Andrew J. M.; McBain, Andrew J.

Abstract

Wound debridement samples and contralateral (healthy) skin swabs acquired from 26 patients attending a specialist foot clinic were analyzed by differential isolation and eubacterium-specific PCR-denaturing gradient gel electrophoresis (DGGE) in conjunction with DNA sequencing. Thirteen of 26 wounds harbored pathogens according to culture analyses, with Staphylococcus aureus being the most common (13/13). Candida (1/13), pseudomonas (1/13), and streptococcus (7/13) were less prevalent. Contralateral skin was associated with comparatively low densities of bacteria, and overt pathogens were not detected. According to DGGE analyses, all wounds contained significantly greater eubacterial diversity than contralateral skin (P

Citation

Oates, A., Bowling, F. L., Boulton, A. J. M., & McBain, A. J. (2012). Molecular and culture-based assessment of the microbial diversity of diabetic chronic foot wounds and contralateral skin sites. Journal of clinical microbiology, 50(7), 2263-2271. https://doi.org/10.1128/JCM.06599-11

• Journal Article Type: Article
• Acceptance Date: Apr 26, 2012
• Online Publication Date: May 2, 2012
• Publication Date: Jul 1, 2012
• Deposit Date: Jan 16, 2019
• Journal: Journal of Clinical Microbiology
• Print ISSN: 0095-1137
• Publisher: American Society for Microbiology
• Peer Reviewed: Peer Reviewed
• Volume: 50
• Issue: 7
• Pages: 2263-2271
• DOI: https://doi.org/10.1128/JCM.06599-11
• Keywords: Microbiology (medical)
• Public URL: https://hull-repository.worktribe.com/output/1217443
• Publisher URL: https://jcm.asm.org/content/50/7/2263/article-info