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Faucicola mancuniensis gen. nov., sp. nov., isolated from the human oropharynx

Humphreys, G. J.; Oates, A.; Ledder, R. G.; McBain, A. J.

Authors

G. J. Humphreys

A. Oates

R. G. Ledder

A. J. McBain



Abstract

An aerobic, Gram-stain-negative, non-motile coccus, designated strain GVCNT2T, was isolated from the tonsils of a healthy adult female. Cells were oxidase- and catalase-positive, positive for the production of esterase (C4), esterase lipase (C8) and leucine arylamidase, and weakly positive for naphthol-AS-BI-phosphohydrolase and alkaline phosphatase. Cells were also capable of hydrolysing DNA. Growth was observed at 20–37 °C and in the presence of up to 1.5 % NaCl. Phylogenetic analysis of near full-length 16S rRNA gene sequences indicated that the strain exhibited closest sequence similarity to Moraxella boevrei ATCC 700022T (94.68 %) and an uncultured, unspeciated bacterial clone (strain S12-08; 99 %). The major fatty acids were C18 : 1ω9c, C18 : 0, C16 : 0 and C16 : 1ω6c/C16 : 1ω7c. The DNA G+C content of strain GVCNT2T was 40.7 mol%. The major respiratory quinone identified was Q-8. Strain GVCNT2T exhibited a comparable phenotypic profile to other members of the genus Moraxella but could be distinguished based on its ability to produce acid (weakly) from d-glucose, melibiose, l-arabinose and rhamnose and on its ability to hydrolyse DNA. On the basis of phenotypic and phylogenetic differences from other members of the family Moraxellaceae , strain GVCNT2T is considered to represent a novel species of a new genus, for which the name Faucicola mancuniensis gen. nov., sp. nov. is proposed. The type strain of Faucicola mancuniensis is GVCNT2T ( = DSM 28411T = NCIMB 14946T).

Journal Article Type Article
Publication Date Jan 1, 2015
Journal INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
Print ISSN 1466-5026
Electronic ISSN 1466-5034
Publisher Microbiology Society
Peer Reviewed Peer Reviewed
Volume 65
Issue Pt 1
Pages 11-14
APA6 Citation Humphreys, G. J., Oates, A., Ledder, R. G., & McBain, A. J. (2015). Faucicola mancuniensis gen. nov., sp. nov., isolated from the human oropharynx. International Journal of Systematic and Evolutionary Microbiology, 65(Pt 1), 11-14. https://doi.org/10.1099/ijs.0.066837-0
DOI https://doi.org/10.1099/ijs.0.066837-0
Keywords New Taxa - Proteobacteria
Publisher URL https://ijs.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.066837-0
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