Long-term imaging of calcium dynamics using genetically encoded calcium indicators and automatic tracking of cultured cells

Razlivanov, Igor; Liew, Teresa; Moore, Eira Watts; Al-Kathiri, Alaa; Bartram, Tayma; Kuvshinov, Dmitriy; Nikolaev, Anton

Abstract

© 2018 Future Science. All rights reserved. Calcium dynamics is crucial for many signaling pathways and cell functions. Understanding how calcium regulates cell function often requires long-term imaging of calcium dynamics. Here we report a methodological approach of long-term (5–10 h) imaging of calcium dynamics in cultured cells. The approach links calcium imaging using genetically encoded calcium indicators and semi-automatic tracking of individual cells. It can be used in a large variety of situations, ranging from the role of calcium in biological processes to cell heterogeneity and screening of drugs modifying signaling pathways.

Citation

Razlivanov, I., Liew, T., Moore, E. W., Al-Kathiri, A., Bartram, T., Kuvshinov, D., & Nikolaev, A. (2018). Long-term imaging of calcium dynamics using genetically encoded calcium indicators and automatic tracking of cultured cells. BioTechniques, 65(1), 37-39. https://doi.org/10.2144/btn-2018-0024

• Journal Article Type: Article
• Acceptance Date: May 28, 2019
• Online Publication Date: Jul 17, 2018
• Publication Date: Jul 17, 2018
• Deposit Date: Jun 11, 2019
• Publicly Available Date: Jun 12, 2019
• Journal: BioTechniques
• Print ISSN: 0736-6205
• Publisher: Future Medicine
• Peer Reviewed: Peer Reviewed
• Volume: 65
• Issue: 1
• Pages: 37-39
• DOI: https://doi.org/10.2144/btn-2018-0024
• Keywords: Automatic cell tracking; Calcium dynamics; Cultured cells; GCaMP5
• Public URL: https://hull-repository.worktribe.com/output/1974457
• Publisher URL: https://www.future-science.com/doi/10.2144/btn-2018-0024
• Additional Information: This work is licensed under the Creative Commons Attribution 4.0 License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
• Contract Date: Jun 11, 2019