Long-term imaging of calcium dynamics using genetically encoded calcium indicators and automatic tracking of cultured cells
Razlivanov, Igor; Liew, Teresa; Moore, Eira Watts; Al-Kathiri, Alaa; Bartram, Tayma; Kuvshinov, Dmitriy; Nikolaev, Anton
Eira Watts Moore
© 2018 Future Science. All rights reserved. Calcium dynamics is crucial for many signaling pathways and cell functions. Understanding how calcium regulates cell function often requires long-term imaging of calcium dynamics. Here we report a methodological approach of long-term (5–10 h) imaging of calcium dynamics in cultured cells. The approach links calcium imaging using genetically encoded calcium indicators and semi-automatic tracking of individual cells. It can be used in a large variety of situations, ranging from the role of calcium in biological processes to cell heterogeneity and screening of drugs modifying signaling pathways.
|Journal Article Type||Article|
|Publication Date||Jul 17, 2018|
|Peer Reviewed||Peer Reviewed|
|APA6 Citation||Razlivanov, I., Liew, T., Moore, E. W., Al-Kathiri, A., Bartram, T., Kuvshinov, D., & Nikolaev, A. (2018). Long-term imaging of calcium dynamics using genetically encoded calcium indicators and automatic tracking of cultured cells. BioTechniques, 65(1), 37-39. https://doi.org/10.2144/btn-2018-0024|
|Keywords||Automatic cell tracking; Calcium dynamics; Cultured cells; GCaMP5|
|Additional Information||This work is licensed under the Creative Commons Attribution 4.0 License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/|
This work is licensed under the Creative Commons Attribution 4.0 License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
You might also like
Maintenance of head and neck tumor on-chip: gateway to personalized treatment?