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Long-term imaging of calcium dynamics using genetically encoded calcium indicators and automatic tracking of cultured cells

Razlivanov, Igor; Liew, Teresa; Moore, Eira Watts; Al-Kathiri, Alaa; Bartram, Tayma; Kuvshinov, Dmitriy; Nikolaev, Anton

Authors

Igor Razlivanov

Teresa Liew

Eira Watts Moore

Alaa Al-Kathiri

Tayma Bartram

Dmitriy Kuvshinov

Anton Nikolaev



Abstract

© 2018 Future Science. All rights reserved. Calcium dynamics is crucial for many signaling pathways and cell functions. Understanding how calcium regulates cell function often requires long-term imaging of calcium dynamics. Here we report a methodological approach of long-term (5–10 h) imaging of calcium dynamics in cultured cells. The approach links calcium imaging using genetically encoded calcium indicators and semi-automatic tracking of individual cells. It can be used in a large variety of situations, ranging from the role of calcium in biological processes to cell heterogeneity and screening of drugs modifying signaling pathways.

Citation

Razlivanov, I., Liew, T., Moore, E. W., Al-Kathiri, A., Bartram, T., Kuvshinov, D., & Nikolaev, A. (2018). Long-term imaging of calcium dynamics using genetically encoded calcium indicators and automatic tracking of cultured cells. BioTechniques, 65(1), 37-39. https://doi.org/10.2144/btn-2018-0024

Journal Article Type Article
Acceptance Date May 28, 2019
Online Publication Date Jul 17, 2018
Publication Date Jul 17, 2018
Deposit Date Jun 11, 2019
Publicly Available Date Jun 12, 2019
Journal BioTechniques
Print ISSN 0736-6205
Electronic ISSN 1940-9818
Publisher Future Medicine
Peer Reviewed Peer Reviewed
Volume 65
Issue 1
Pages 37-39
DOI https://doi.org/10.2144/btn-2018-0024
Keywords Automatic cell tracking; Calcium dynamics; Cultured cells; GCaMP5
Public URL https://hull-repository.worktribe.com/output/1974457
Publisher URL https://www.future-science.com/doi/10.2144/btn-2018-0024
Additional Information This work is licensed under the Creative Commons Attribution 4.0 License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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