Skip to main content

Research Repository

Advanced Search

Biosensor for determining average iron content of ferritin by measuring its optical dispersion

Gupta, Ruchi; Alamrani, Nasser A.; Greenway, Gillian M.; Pamme, Nicole; Goddard, Nicholas J.

Authors

Ruchi Gupta

Nasser A. Alamrani

Gillian M. Greenway

Nicholas J. Goddard



Abstract

© 2020 SPIE. Average iron content of ferritin has a potential to serve as a biomarker for early identification of high-risk trauma patients at point-of-care (PoC). Appropriate therapies can then be administered to reduce morbidity and mortality. Currently, protein and iron levels are measured separately using enzyme-linked immunosorbent assay (ELISA) and UV or atomic absorption spectroscopy (AAS) respectively, but the use of two completely different methods adds to the complexity and analysis time of the combined measurement. As a result, these methods are unsuitable for PoC analysis. To address this gap, we report a biosensor for measuring the average iron content of ferritin in a single step. The biosensor was based on a dye-doped leaky waveguide (LW), which operates in the entire visible wavelength range, and hence allowed the measurement of differences in the optical dispersion of ferritin and apoferritin to determine the average iron content of the protein. The LW biosensor comprised a 1.54 micron thick mesoporous chitosan slab waveguide with immobilized antibodies against ferritin/apoferritin to measure the optical dispersion of 110 nM protein. Based on the baseline noise, the limit of detection for this method is ∼700 pM for ferritin/apoferritin. The biosensor has a significant potential for PoC measurement of the average iron content of serum ferritin and, in future, the total protein cencentration.

Citation

Gupta, R., Alamrani, N. A., Greenway, G. M., Pamme, N., & Goddard, N. J. (2020). Biosensor for determining average iron content of ferritin by measuring its optical dispersion. Proceedings of SPIE, 11235, https://doi.org/10.1117/12.2542162

Journal Article Type Conference Paper
Acceptance Date Jan 1, 2020
Online Publication Date Feb 21, 2020
Publication Date Feb 21, 2020
Deposit Date Dec 8, 2020
Publicly Available Date Jan 20, 2021
Journal Proceedings of SPIE
Print ISSN 0277-786X
Publisher Society of Photo-optical Instrumentation Engineers
Peer Reviewed Peer Reviewed
Volume 11235
ISBN 9781510632332
DOI https://doi.org/10.1117/12.2542162
Keywords Waveguide; Biosensor; Chitosan; Mesoporous; Optical dispersion; Ferritin; Point-of-care; Microfluidic
Public URL https://hull-repository.worktribe.com/output/3497454
Publisher URL https://www.spiedigitallibrary.org/conference-proceedings-of-spie/11235/2542162/Biosensor-for-determining-average-iron-content-of-ferritin-by-measuring/10.1117/12.2542162.short

Files

Article (1.2 Mb)
PDF

Copyright Statement
© (2020) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE).



You might also like



Downloadable Citations