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The mitochondrial phosphate carrier TbMCP11 is essential for mitochondrial function in the procyclic form of Trypanosoma brucei

Gao, Fei; Voncken, Frank; Colasante, Claudia

Authors

Fei Gao

Frank Voncken

Claudia Colasante



Abstract

Conserved amongst all eukaryotes is a family of mitochondrial carrier proteins (SLC25A) responsible for the import of various solutes across the inner mitochondrial membrane. We previously reported that the human parasite Trypanosoma brucei possesses 26 SLC25A proteins (TbMCPs) amongst which two, TbMCP11 and TbMCP8, were predicted to function as phosphate importers. The transport of inorganic phosphate into the mitochondrion is a prerequisite to drive ATP synthesis by substrate level and oxidative phosphorylation and thus crucial for cell viability. In this paper we describe the functional characterization of TbMCP11. In procyclic form T. brucei, the RNAi of TbMCP11 blocked ATP synthesis on mitochondrial substrates, caused a drop of the mitochondrial oxygen consumption and drastically reduced cell viability. The functional complementation in yeast and mitochondrial swelling experiments suggested a role for TbMCP11 as inorganic phosphate carrier. Interestingly, procyclic form T. brucei cells in which TbMCP11 was depleted displayed an inability to either replicate or divide the kinetoplast DNA, which resulted in a severe cytokinesis defect.

Journal Article Type Article
Journal Molecular and Biochemical Parasitology
Print ISSN 0166-6851
Publisher Elsevier
Peer Reviewed Peer Reviewed
Article Number 111275
APA6 Citation Gao, F., Voncken, F., & Colasante, C. (in press). The mitochondrial phosphate carrier TbMCP11 is essential for mitochondrial function in the procyclic form of Trypanosoma brucei. Molecular and Biochemical Parasitology, https://doi.org/10.1016/j.molbiopara.2020.111275
DOI https://doi.org/10.1016/j.molbiopara.2020.111275
Keywords Mitochondrial carrier family; Energy metabolism; Trypanosoma brucei; Cytokinesis; Phosphate transport; Mitochondrial membrane potential
Publisher URL https://www.sciencedirect.com/science/article/pii/S0166685120300396?via%3Dihub#!
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