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Characterisation of a mitochondrial iron transporter of the pathogen Trypanosoma brucei

Zheng, Fuli; Colasante, Claudia; Voncken, Frank

Authors

Fuli Zheng

Claudia Colasante

Frank Voncken



Abstract

Similar to higher eukaryotes, the protist parasite T. brucei harbours several iron-containing proteins that regulate DNA and protein processing, oxidative stress defence and mitochondrial respiration. The synthesis of these proteins occurs either in the cytoplasm or within the mitochondrion. For mitochondrial iron cluster protein synthesis, iron needs to be transported across the solute impermeable mitochondrial membrane. In T. brucei we previously identified 24 mitochondrial carrier proteins (TbMCPs) sharing conserved structural and functional features with those from higher eukaryotes. One of these carriers (TbMCP17) displayed high similarity with the iron carriers MRS3, MRS4 from yeast and mitoferrin from mammals, insects and plants. In the present study we demonstrated that TbMCP17 functions as an iron carrier by complementation studies using MRS3/4-deficient yeast. Depletion of TbMCP17 in procyclic form T. brucei resulted in growth deficiency, increased sensitivity to iron deprivation, and lowered mitochondrial iron content. Taken together our results suggest that TbMCP17 functions as a mitochondrial iron transporter in the parasite T. brucei.

Journal Article Type Article
Publication Date 2019-10
Journal Molecular and Biochemical Parasitology
Print ISSN 0166-6851
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 233
Pages 111221
APA6 Citation Zheng, F., Colasante, C., & Voncken, F. (2019). Characterisation of a mitochondrial iron transporter of the pathogen Trypanosoma brucei. Molecular and Biochemical Parasitology, 233, 111221. https://doi.org/10.1016/j.molbiopara.2019.111221
DOI https://doi.org/10.1016/j.molbiopara.2019.111221
Keywords Mitochondrial transport; Iron metabolism; Trypanosoma brucei; Mitochondrial carrier family-proteins
Publisher URL https://www.sciencedirect.com/science/article/pii/S0166685119301008?via%3Dihub
Additional Information This article is maintained by: Elsevier; Article Title: Characterisation of a mitochondrial iron transporter of the pathogen Trypanosoma brucei; Journal Title: Molecular and Biochemical Parasitology; CrossRef DOI link to publisher maintained version: https://doi.org/10.1016/j.molbiopara.2019.111221; Content Type: article; Copyright: © 2019 Elsevier B.V. All rights reserved.

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Copyright Statement
©2019, Elsevier. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/





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