Fuli Zheng
Characterisation of a mitochondrial iron transporter of the pathogen Trypanosoma brucei
Zheng, Fuli; Colasante, Claudia; Voncken, Frank
Authors
Claudia Colasante
Dr Frank Voncken Frank.Voncken@hyms.ac.uk
Lecturer in Medical Microbiology, and Biomedical and Forensic Sciences. Biosafety Practitioner and Advisor.
Abstract
Similar to higher eukaryotes, the protist parasite T. brucei harbours several iron-containing proteins that regulate DNA and protein processing, oxidative stress defence and mitochondrial respiration. The synthesis of these proteins occurs either in the cytoplasm or within the mitochondrion. For mitochondrial iron cluster protein synthesis, iron needs to be transported across the solute impermeable mitochondrial membrane. In T. brucei we previously identified 24 mitochondrial carrier proteins (TbMCPs) sharing conserved structural and functional features with those from higher eukaryotes. One of these carriers (TbMCP17) displayed high similarity with the iron carriers MRS3, MRS4 from yeast and mitoferrin from mammals, insects and plants. In the present study we demonstrated that TbMCP17 functions as an iron carrier by complementation studies using MRS3/4-deficient yeast. Depletion of TbMCP17 in procyclic form T. brucei resulted in growth deficiency, increased sensitivity to iron deprivation, and lowered mitochondrial iron content. Taken together our results suggest that TbMCP17 functions as a mitochondrial iron transporter in the parasite T. brucei.
Citation
Zheng, F., Colasante, C., & Voncken, F. (2019). Characterisation of a mitochondrial iron transporter of the pathogen Trypanosoma brucei. Molecular and Biochemical Parasitology, 233, Article 111221. https://doi.org/10.1016/j.molbiopara.2019.111221
Journal Article Type | Article |
---|---|
Acceptance Date | Sep 13, 2019 |
Online Publication Date | Sep 19, 2019 |
Publication Date | 2019-10 |
Deposit Date | Oct 25, 2019 |
Publicly Available Date | Sep 20, 2020 |
Journal | Molecular and Biochemical Parasitology |
Print ISSN | 0166-6851 |
Publisher | Elsevier |
Peer Reviewed | Peer Reviewed |
Volume | 233 |
Article Number | 111221 |
DOI | https://doi.org/10.1016/j.molbiopara.2019.111221 |
Keywords | Mitochondrial transport; Iron metabolism; Trypanosoma brucei; Mitochondrial carrier family-proteins |
Public URL | https://hull-repository.worktribe.com/output/3001373 |
Publisher URL | https://www.sciencedirect.com/science/article/pii/S0166685119301008?via%3Dihub |
Additional Information | This article is maintained by: Elsevier; Article Title: Characterisation of a mitochondrial iron transporter of the pathogen Trypanosoma brucei; Journal Title: Molecular and Biochemical Parasitology; CrossRef DOI link to publisher maintained version: https://doi.org/10.1016/j.molbiopara.2019.111221; Content Type: article; Copyright: © 2019 Elsevier B.V. All rights reserved. |
Contract Date | Oct 28, 2019 |
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Copyright Statement
©2019, Elsevier. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
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