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Pre-Clinical Assessment of Single-Use Negative Pressure Wound Therapy During In Vivo Porcine Wound Healing

Brownhill, Varuni; Huddleston, Elizabeth; Bell, Andrea; Hart, Jeffery; Webster, Iain; Hardman, Matthew; Wilkinson, Holly


Varuni Brownhill

Elizabeth Huddleston

Andrea Bell

Jeffery Hart

Iain Webster


Objective: Traditional negative pressure wound therapy systems can be large and cumbersome, limiting patient mobility and adversely affecting quality of life. PICO™, a no canister single-use system offers a lightweight, portable alternative to traditional negative pressure wound therapy, with improved clinical performance. The aim of this study was to determine the potential mechanism(s) of action of single-use negative pressure wound therapy versus traditional negative pressure wound therapy.
Approach: Single-use negative pressure wound therapy and traditional negative pressure wound therapy were applied to in in vivo porcine excisional wound model, following product use guidelines. Macroscopic, histological and biochemical analyses were performed at defined healing time-points to assess multiple aspects of the healing response.
Results: Wounds treated with single-use negative pressure displayed greater wound closure and increased re-epithelialisation versus those treated with traditional negative pressure. The resulting granulation tissue was more advanced with fewer neutrophils, reduced inflammatory markers, more mature collagen and no wound filler-associated foreign body reactions. Of note, single-use negative pressure therapy failed to induce wound edge epithelial hyperproliferation, while traditional negative pressure therapy compromised peri-wound skin, which remained inflamed with high transepidermal water loss; features not observed following single-use treatment.
Innovation: Single-use negative pressure was identified to improve multiple aspects of healing versus traditional negative pressure treatment.
Conclusion: This study provides important new insight into the differing mode of action of single-use versus traditional negative pressure and may go some way to explain the improved clinical outcomes observed with single use negative pressure therapy.


Brownhill, V., Huddleston, E., Bell, A., Hart, J., Webster, I., Hardman, M., & Wilkinson, H. (2021). Pre-Clinical Assessment of Single-Use Negative Pressure Wound Therapy During In Vivo Porcine Wound Healing. Advances in wound care, 10(7), 345-356.

Journal Article Type Article
Acceptance Date Jun 26, 2020
Online Publication Date Jul 7, 2020
Publication Date May 26, 2021
Deposit Date Jun 27, 2020
Publicly Available Date Jan 8, 2021
Journal Advances in wound care
Print ISSN 2162-1918
Peer Reviewed Peer Reviewed
Volume 10
Issue 7
Pages 345-356
Keywords NPWT; Porcine; Wound healing; Pressure
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Copyright Statement
© Varuni R. Brownhill et al., 2020; Published by Mary Ann Liebert, Inc. This Open Access article is distributed under the terms of the Creative Commons Attribution Noncommercial License [CC-BY-NC] ( which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and the source are cited.

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