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Microfluidic-Based Electrochemical Immunosensing of Ferritin

Garg, Mayank; Christensen, Martin Gedsted; Iles, Alexander; Sharma, Amit L.; Singh, Suman; Pamme, Nicole

Authors

Mayank Garg

Martin Gedsted Christensen

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Dr Alex Iles a.Iles@hull.ac.uk
Experimental Officer - Lab on a Chip

Amit L. Sharma

Suman Singh



Abstract

Ferritin is a clinically important biomarker which reflects the state of iron in the body and is directly involved with anemia. Current methods available for ferritin estimation are generally not portable or they do not provide a fast response. To combat these issues, an attempt was made for lab-on-a-chip-based electrochemical detection of ferritin, developed with an integrated electrochemically active screen-printed electrode (SPE), combining nanotechnology, microfluidics, and electrochemistry. The SPE surface was modified with amine-functionalized graphene oxide to facilitate the binding of ferritin antibodies on the electrode surface. The functionalized SPE was embedded in the microfluidic flow cell with a simple magnetic clamping mechanism to allow continuous electrochemical detection of ferritin. Ferritin detection was accomplished via cyclic voltammetry with a dynamic linear range from 7.81 to 500 ng·mL-1 and an LOD of 0.413 ng·mL-1. The sensor performance was verified with spiked human serum samples. Furthermore, the sensor was validated by comparing its response with the response of the conventional ELISA method. The current method of microfluidic flow cell-based electrochemical ferritin detection demonstrated promising sensitivity and selectivity. This confirmed the plausibility of using the reported technique in point-of-care testing applications at a much faster rate than conventional techniques.

Citation

Garg, M., Christensen, M. G., Iles, A., Sharma, A. L., Singh, S., & Pamme, N. (2020). Microfluidic-Based Electrochemical Immunosensing of Ferritin. Biosensors, 10(8), https://doi.org/10.3390/bios10080091

Journal Article Type Article
Acceptance Date Aug 2, 2020
Online Publication Date Aug 5, 2020
Publication Date Aug 5, 2020
Deposit Date Aug 6, 2020
Publicly Available Date Aug 6, 2020
Journal Biosensors
Print ISSN 2079-6374
Electronic ISSN 2079-6374
Publisher MDPI
Peer Reviewed Peer Reviewed
Volume 10
Issue 8
Article Number 91
DOI https://doi.org/10.3390/bios10080091
Keywords Clinical Biochemistry; General Medicine
Public URL https://hull-repository.worktribe.com/output/3552311
Publisher URL https://www.mdpi.com/2079-6374/10/8/91

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