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Antibiofilm activity of heather and manuka honeys and antivirulence potential of some of their constituents on the DsbA1 enzyme of pseudomonas aeruginosa

Shirlaw, Oscar; Billah, Zara; Attar, Baraa; Hughes, Lisa; Qasaymeh, Rana M.; Seidel, Veronique; Efthimiou, Georgios

Authors

Oscar Shirlaw

Zara Billah

Baraa Attar

Lisa Hughes

Rana M. Qasaymeh

Veronique Seidel



Abstract

Heather honey was tested for its effect on the formation of biofilms by Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Klebsiella pneumoniae, Enterococcus faecalis, Salmonella Enteriditis and Acinetobacter baumanii in comparison with Manuka honey. At 0.25 mg/mL, Heather honey inhibited biofilm formation in S. aureus, A. baumanii, E. coli, S. Enteriditis and P. aeruginosa, but promoted the growth of E. faecalis and K. pneumoniae biofilms. Manuka honey inhibited biofilm formation in K. pneumoniae, E. faecalis, and S. Enteriditis, A. baumanii, E. coli and P. aeruginosa, but promoted S. aureus biofilm formation. Molecular docking with Autodock Vina was performed to calculate the predictive binding affinities and ligand efficiencies of Manuka and Heather honey constituents for PaDsbA1, the main enzyme controlling the correct folding of virulence proteins in Pseudomonas aeruginosa. A number of constituents, including benzoic acid and methylglyoxal, present in Heather and/or Manuka honey, revealed high ligand efficiencies for the target enzyme. This helps support, to some extent, the decrease in P. aeruginosa biofilm formation observed for such honeys.

Citation

Shirlaw, O., Billah, Z., Attar, B., Hughes, L., Qasaymeh, R. M., Seidel, V., & Efthimiou, G. (2020). Antibiofilm activity of heather and manuka honeys and antivirulence potential of some of their constituents on the DsbA1 enzyme of pseudomonas aeruginosa. Antibiotics, 9(12), 1-12. https://doi.org/10.3390/antibiotics9120911

Journal Article Type Article
Acceptance Date Dec 10, 2020
Online Publication Date Dec 15, 2020
Publication Date 2020-12
Deposit Date Dec 18, 2020
Publicly Available Date Dec 18, 2020
Journal Antibiotics
Print ISSN 2079-6382
Electronic ISSN 2079-6382
Publisher MDPI
Peer Reviewed Peer Reviewed
Volume 9
Issue 12
Article Number 911
Pages 1-12
DOI https://doi.org/10.3390/antibiotics9120911
Keywords Antibiofilm; Antivirulence; Heather honey; Manuka honey; Pseudomonas aeruginosa
Public URL https://hull-repository.worktribe.com/output/3679764
Publisher URL https://www.mdpi.com/2079-6382/9/12/911

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Publisher Licence URL
https://creativecommons.org/licenses/by/4.0/

Copyright Statement
© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).



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