Antibiofilm activity of heather and manuka honeys and antivirulence potential of some of their constituents on the DsbA1 enzyme of pseudomonas aeruginosa
Shirlaw, Oscar; Billah, Zara; Attar, Baraa; Hughes, Lisa; Qasaymeh, Rana M.; Seidel, Veronique; Efthimiou, Georgios
Rana M. Qasaymeh
Dr Georgios Efthimiou G.Efthimiou@hull.ac.uk
Lecturer in Microbiology
Heather honey was tested for its effect on the formation of biofilms by Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Klebsiella pneumoniae, Enterococcus faecalis, Salmonella Enteriditis and Acinetobacter baumanii in comparison with Manuka honey. At 0.25 mg/mL, Heather honey inhibited biofilm formation in S. aureus, A. baumanii, E. coli, S. Enteriditis and P. aeruginosa, but promoted the growth of E. faecalis and K. pneumoniae biofilms. Manuka honey inhibited biofilm formation in K. pneumoniae, E. faecalis, and S. Enteriditis, A. baumanii, E. coli and P. aeruginosa, but promoted S. aureus biofilm formation. Molecular docking with Autodock Vina was performed to calculate the predictive binding affinities and ligand efficiencies of Manuka and Heather honey constituents for PaDsbA1, the main enzyme controlling the correct folding of virulence proteins in Pseudomonas aeruginosa. A number of constituents, including benzoic acid and methylglyoxal, present in Heather and/or Manuka honey, revealed high ligand efficiencies for the target enzyme. This helps support, to some extent, the decrease in P. aeruginosa biofilm formation observed for such honeys.
Shirlaw, O., Billah, Z., Attar, B., Hughes, L., Qasaymeh, R. M., Seidel, V., & Efthimiou, G. (2020). Antibiofilm activity of heather and manuka honeys and antivirulence potential of some of their constituents on the DsbA1 enzyme of pseudomonas aeruginosa. Antibiotics, 9(12), 1-12. https://doi.org/10.3390/antibiotics9120911
|Journal Article Type||Article|
|Acceptance Date||Dec 10, 2020|
|Online Publication Date||Dec 15, 2020|
|Deposit Date||Dec 18, 2020|
|Publicly Available Date||Dec 18, 2020|
|Peer Reviewed||Peer Reviewed|
|Keywords||Antibiofilm; Antivirulence; Heather honey; Manuka honey; Pseudomonas aeruginosa|
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© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).