Transition in sexual system and sex chromosome evolution in the tadpole shrimp Triops cancriformis

Mathers, T. C.; Hammond, R. L.; Jenner, Ronald; Hänfling, B.; Atkins, Jennifer; Gómez, A.

Abstract

© 2015 Macmillan Publishers Limited All rights reserved. Transitions in sexual system and reproductive mode may affect the course of sex chromosome evolution, for instance by altering the strength of sexually antagonistic selection. However, there have been few studies of sex chromosomes in systems where such transitions have been documented. The European tadpole shrimp, Triops cancriformis, has undergone a transition from dioecy to androdioecy (a sexual system where hermaphrodites and males coexist), offering an excellent opportunity to test the impact of this transition on the evolution of sex chromosomes. To identify sex-linked markers, to understand mechanisms of sex determination and to investigate differences between sexual systems, we carried out a genome-wide association study using restriction site-associated DNA sequencing (RAD-seq) of 47 males, females and hermaphrodites from one dioecious and one androdioecious population. We analysed 22.9 Gb of paired-end sequences and identified and scored > 3000 high coverage novel genomic RAD markers. Presence-absence of markers, single-nucleotide polymorphism association and read depth identified 52 candidate sex-linked markers. We show that sex is genetically determined in T. cancriformis, with a ZW system conserved across dioecious and androdioecious populations and that hermaphrodites have likely evolved from females. We also show that the structure of the sex chromosomes differs strikingly, with a larger sex-linked region in the dioecious population compared with the androdioecious population.

Citation

Mathers, T. C., Hammond, R. L., Jenner, R., Hänfling, B., Atkins, J., & Gómez, A. (2015). Transition in sexual system and sex chromosome evolution in the tadpole shrimp Triops cancriformis. Heredity, 115(1), 37-46. https://doi.org/10.1038/hdy.2015.10

• Journal Article Type: Article
• Acceptance Date: Jan 14, 2015
• Online Publication Date: Mar 11, 2015
• Publication Date: 2015-01
• Deposit Date: May 19, 2015
• Publicly Available Date: May 19, 2015
• Journal: Heredity
• Print ISSN: 0018-067X
• Electronic ISSN: 1365-2540
• Publisher: Nature Publishing Group
• Peer Reviewed: Peer Reviewed
• Volume: 115
• Issue: 1
• Pages: 37-46
• DOI: https://doi.org/10.1038/hdy.2015.10
• Keywords: Gonochoric, Hermaphroditism, Sexually antagonistic selection, RAD-seq, Androdioecy, Linkage
• Public URL: https://hull-repository.worktribe.com/output/373929
• Publisher URL: http://www.nature.com/hdy/journal/vaop/ncurrent/abs/hdy201510a.html
• Additional Information: Copy of article first published in: Heredity, 2015, v.115.