A novel fluorescent "turn-on" chemosensor for nanomolar detection of Fe(III) from aqueous solution and its application in living cells imaging

Prior, Timothy.; Redshaw, Carl.; Chen, Lingxin.; Mahulikar, Pramod.; Nandre, Jitendra.; Patil, Samadhan.; Patil, Umesh.; Patil, Vijay.; Sahoo, Suban.; Yu, Fabiao.; Nandre, J..; Patil, S..; Patil, V..; Yu, F..; Chen, L..; Sahoo, S..; Mahulikar, P..; Patil, U..

doi:10.1016/j.bios.2014.06.017

Authors

U. Patil

S. Patil

P. Mahulikar

S. Sahoo

L. Chen

F. Yu

V. Patil

J. Nandre

Jitendra Nandre

Samadhan Patil

Vijay Patil

Fabiao Yu

Lingxin Chen

Suban Sahoo

Dr Timothy Prior T.Prior@hull.ac.uk
Lecturer in Inorganic Chemistry

Professor Carl Redshaw C.Redshaw@hull.ac.uk
Professor of Inorganic Materials Chemistry and REF Lead for Chemistry

Pramod Mahulikar

Umesh Patil

Abstract

An electronically active and spectral sensitive fluorescent “turn-on” chemosensor (BTP-1) based on the benzo-thiazolo-pyrimidine unit was designed and synthesized for the highly selective and sensitive detection of Fe³⁺ from aqueous medium. With Fe³⁺, the sensor BTP-1 showed a remarkable fluorescence enhancement at 554 nm (λex=314 nm) due to the inhibition of photo-induced electron transfer. The sensor formed a host-guest complex in 1:1 stoichiometry with the detection limit down to 0.74 nM. Further, the sensor was successfully utilized for the qualitative and quantitative intracellular detection of Fe³⁺ in two liver cell lines i.e., HepG2 cells (human hepatocellular liver carcinoma cell line) and HL-7701 cells (human normal liver cell line) by a confocal imaging technique.

Journal Article Type	Article
Publication Date	Nov 15, 2014
Journal	Biosensors & bioelectronics
Print ISSN	0956-5663
Electronic ISSN	1873-4235
Publisher	Elsevier
Peer Reviewed	Peer Reviewed
Volume	61
Issue	November
Pages	612-617
Institution Citation	Nandre, J., Patil, S., Patil, V., Yu, F., Chen, L., Sahoo, S., …Patil, U. (2014). A novel fluorescent "turn-on" chemosensor for nanomolar detection of Fe(III) from aqueous solution and its application in living cells imaging. Biosensors & bioelectronics, 61(November), 612-617. https://doi.org/10.1016/j.bios.2014.06.017
DOI	https://doi.org/10.1016/j.bios.2014.06.017
Keywords	Fluorescent “turn-on” sensor, Benzo-thiazolo-pyrimidine, Fe³⁺, ICT, PET, Live cells imaging
Publisher URL	http://www.sciencedirect.com/science/article/pii/S0956566314004308
Copyright Statement	© 2014, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/
Additional Information	Author's accepted manuscript of article published in: Biosensors & bioelectronics, 2014, v.61, November.

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Copyright Statement
© 2014, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/