A novel fluorescent "turn-on" chemosensor for nanomolar detection of Fe(III) from aqueous solution and its application in living cells imaging

Patil, U.; Patil, S.; Mahulikar, P.; Sahoo, S.; Chen, L.; Yu, F.; Patil, V.; Nandre, J.; Nandre, Jitendra; Patil, Samadhan; Patil, Vijay; Yu, Fabiao; Chen, Lingxin; Sahoo, Suban; Prior, Timothy; Redshaw, Carl; Mahulikar, Pramod; Patil, Umesh

Abstract

An electronically active and spectral sensitive fluorescent “turn-on” chemosensor (BTP-1) based on the benzo-thiazolo-pyrimidine unit was designed and synthesized for the highly selective and sensitive detection of Fe³⁺ from aqueous medium. With Fe³⁺, the sensor BTP-1 showed a remarkable fluorescence enhancement at 554 nm (λex=314 nm) due to the inhibition of photo-induced electron transfer. The sensor formed a host-guest complex in 1:1 stoichiometry with the detection limit down to 0.74 nM. Further, the sensor was successfully utilized for the qualitative and quantitative intracellular detection of Fe³⁺ in two liver cell lines i.e., HepG2 cells (human hepatocellular liver carcinoma cell line) and HL-7701 cells (human normal liver cell line) by a confocal imaging technique.

• Journal Article Type: Article
• Publication Date: Nov 15, 2014
• Journal: Biosensors & bioelectronics
• Print ISSN: 0956-5663
• Electronic ISSN: 1873-4235
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 61
• Issue: November
• Pages: 612-617
• Institution Citation: Nandre, J., Patil, S., Patil, V., Yu, F., Chen, L., Sahoo, S., …Patil, U. (2014). A novel fluorescent "turn-on" chemosensor for nanomolar detection of Fe(III) from aqueous solution and its application in living cells imaging. Biosensors & bioelectronics, 61(November), 612-617. https://doi.org/10.1016/j.bios.2014.06.017
• DOI: https://doi.org/10.1016/j.bios.2014.06.017
• Keywords: Fluorescent “turn-on” sensor, Benzo-thiazolo-pyrimidine, Fe³⁺, ICT, PET, Live cells imaging
• Publisher URL: http://www.sciencedirect.com/science/article/pii/S0956566314004308
• Copyright Statement: © 2014, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/
• Additional Information: Author's accepted manuscript of article published in: Biosensors & bioelectronics, 2014, v.61, November.