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A novel fluorescent "turn-on" chemosensor for nanomolar detection of Fe(III) from aqueous solution and its application in living cells imaging

Patil, U.; Patil, S.; Mahulikar, P.; Sahoo, S.; Chen, L.; Yu, F.; Patil, V.; Nandre, J.; Nandre, Jitendra; Patil, Samadhan; Patil, Vijay; Yu, Fabiao; Chen, Lingxin; Sahoo, Suban; Prior, Timothy; Redshaw, Carl; Mahulikar, Pramod; Patil, Umesh

Authors

U. Patil

S. Patil

P. Mahulikar

S. Sahoo

L. Chen

F. Yu

V. Patil

J. Nandre

Jitendra Nandre

Samadhan Patil

Vijay Patil

Fabiao Yu

Lingxin Chen

Suban Sahoo

Professor Carl Redshaw C.Redshaw@hull.ac.uk
Professor of Inorganic Materials Chemistry and REF Lead for Chemistry

Pramod Mahulikar

Umesh Patil

Abstract

An electronically active and spectral sensitive fluorescent “turn-on” chemosensor (BTP-1) based on the benzo-thiazolo-pyrimidine unit was designed and synthesized for the highly selective and sensitive detection of Fe³⁺ from aqueous medium. With Fe³⁺, the sensor BTP-1 showed a remarkable fluorescence enhancement at 554 nm (λex=314 nm) due to the inhibition of photo-induced electron transfer. The sensor formed a host-guest complex in 1:1 stoichiometry with the detection limit down to 0.74 nM. Further, the sensor was successfully utilized for the qualitative and quantitative intracellular detection of Fe³⁺ in two liver cell lines i.e., HepG2 cells (human hepatocellular liver carcinoma cell line) and HL-7701 cells (human normal liver cell line) by a confocal imaging technique.

Journal Article Type Article
Publication Date Nov 15, 2014
Journal Biosensors & bioelectronics
Print ISSN 0956-5663
Electronic ISSN 1873-4235
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 61
Issue November
Pages 612-617
Institution Citation Nandre, J., Patil, S., Patil, V., Yu, F., Chen, L., Sahoo, S., …Patil, U. (2014). A novel fluorescent "turn-on" chemosensor for nanomolar detection of Fe(III) from aqueous solution and its application in living cells imaging. Biosensors & bioelectronics, 61(November), 612-617. https://doi.org/10.1016/j.bios.2014.06.017
DOI https://doi.org/10.1016/j.bios.2014.06.017
Keywords Fluorescent “turn-on” sensor, Benzo-thiazolo-pyrimidine, Fe³⁺, ICT, PET, Live cells imaging
Publisher URL http://www.sciencedirect.com/science/article/pii/S0956566314004308
Copyright Statement © 2014, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/
Additional Information Author's accepted manuscript of article published in: Biosensors & bioelectronics, 2014, v.61, November.

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Copyright Statement
© 2014, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/




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