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Phaseguide assisted liquid lamination for magnetic particle-based assays

Phurimsak, Chayakom; Yildirim, Ender; Tarn, Mark D.; Trietsch, Sebastiaan J.; Hankemeier, Thomas; Pamme, Nicole; Vulto, Paul

Authors

Chayakom Phurimsak

Ender Yildirim

Mark D. Tarn

Sebastiaan J. Trietsch

Thomas Hankemeier

Paul Vulto

Abstract

We have developed a magnetic particle-based assay platform in which functionalised magnetic particles are transferred sequentially through laminated volumes of reagents and washing buffers. Lamination of aqueous liquids is achieved via the use of phaseguide technology; microstructures that control the advancing air–liquid interface of solutions as they enter a microfluidic chamber. This allows manual filling of the device, eliminating the need for external pumping systems, and preparation of the system requires only a few minutes. Here, we apply the platform to two on-chip strategies: (i) a one-step streptavidin–biotin binding assay, and (ii) a two-step C-reactive protein immunoassay. With these, we demonstrate how condensing multiple reaction and washing processes into a single step significantly reduces procedural times, with both assay procedures requiring less than 8 seconds.

Journal Article Type Article
Publication Date Jul 7, 2014
Journal Lab on a chip
Print ISSN 1473-0197
Electronic ISSN 1473-0189
Publisher Royal Society of Chemistry
Peer Reviewed Peer Reviewed
Volume 14
Issue 13
Pages 2334-2343
Institution Citation Phurimsak, C., Yildirim, E., Tarn, M. D., Trietsch, S. J., Hankemeier, T., Pamme, N., & Vulto, P. (2014). Phaseguide assisted liquid lamination for magnetic particle-based assays. Lab on a chip, 14(13), 2334-2343. https://doi.org/10.1039/c4lc00139g
DOI https://doi.org/10.1039/c4lc00139g
Keywords Liquid lamination, Magnetic particle-based assays
Publisher URL http://pubs.rsc.org/en/Content/ArticleLanding/2014/LC/c4lc00139g#!divAbstract
Additional Information : This document is CrossCheck deposited; : Supplementary Information; : The Royal Society of Chemistry has an exclusive publication licence for this journal; OPEN ACCESS: The accepted version of this article will be made freely available after a 12 month embargo period; : Received 31 January 2014; Accepted 25 April 2014; Accepted Manuscript published 25 April 2014; Advance Article published 16 May 2014; Version of Record published 2 June 2014

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