Phaseguide assisted liquid lamination for magnetic particle-based assays

Phurimsak, Chayakom; Yildirim, Ender; Tarn, Mark D.; Trietsch, Sebastiaan J.; Hankemeier, Thomas; Pamme, Nicole; Vulto, Paul

Abstract

We have developed a magnetic particle-based assay platform in which functionalised magnetic particles are transferred sequentially through laminated volumes of reagents and washing buffers. Lamination of aqueous liquids is achieved via the use of phaseguide technology; microstructures that control the advancing air–liquid interface of solutions as they enter a microfluidic chamber. This allows manual filling of the device, eliminating the need for external pumping systems, and preparation of the system requires only a few minutes. Here, we apply the platform to two on-chip strategies: (i) a one-step streptavidin–biotin binding assay, and (ii) a two-step C-reactive protein immunoassay. With these, we demonstrate how condensing multiple reaction and washing processes into a single step significantly reduces procedural times, with both assay procedures requiring less than 8 seconds.

Citation

Phurimsak, C., Yildirim, E., Tarn, M. D., Trietsch, S. J., Hankemeier, T., Pamme, N., & Vulto, P. (2014). Phaseguide assisted liquid lamination for magnetic particle-based assays. Lab on a chip, 14(13), 2334-2343. https://doi.org/10.1039/c4lc00139g

• Journal Article Type: Article
• Acceptance Date: Apr 25, 2014
• Online Publication Date: Apr 25, 2014
• Publication Date: Jul 7, 2014
• Deposit Date: Sep 2, 2015
• Publicly Available Date: Nov 23, 2017
• Journal: Lab on a chip
• Print ISSN: 1473-0197
• Electronic ISSN: 1473-0189
• Publisher: Royal Society of Chemistry
• Peer Reviewed: Peer Reviewed
• Volume: 14
• Issue: 13
• Pages: 2334-2343
• DOI: https://doi.org/10.1039/c4lc00139g
• Keywords: Liquid lamination, Magnetic particle-based assays
• Public URL: https://hull-repository.worktribe.com/output/378590
• Publisher URL: http://pubs.rsc.org/en/Content/ArticleLanding/2014/LC/c4lc00139g#!divAbstract
• Additional Information: : This document is CrossCheck deposited; : Supplementary Information; : The Royal Society of Chemistry has an exclusive publication licence for this journal; OPEN ACCESS: The accepted version of this article will be made freely available after a 12 month embargo period; : Received 31 January 2014; Accepted 25 April 2014; Accepted Manuscript published 25 April 2014; Advance Article published 16 May 2014; Version of Record published 2 June 2014